加味六君子汤含药血清对胃癌细胞BGC-823增殖和凋亡的影响  

Effects of Jia-wei-liu-jun-zi Decoction on Cell Cycle and Apoptosis of Human Gastric Carcinoma Cell Line BGC-823 in vitro

作　　者：胡玉娜[1] 赵晓艳[1] 倪振华[1] 孙健[1] 季青[1] 张隆[1] 刘丽丽[1] 康向东[1] 何建成[2] 

机构地区：[1]上海中医药大学附属普陀医院,上海200062 [2]上海中医药大学

出　　处：《浙江中西医结合杂志》2010年第4期205-207,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:观察加味六君子汤含药血清对胃癌BGC-823细胞增殖和凋亡的影响。方法:以加味六君子汤不同浓度含药血清培养BGC-823细胞后,以MTT法检测对细胞增殖的影响,流式细胞仪检测细胞周期和细胞凋亡变化。结果:浓度为10%、15%、20%、30%、40%的含药血清作用于BGC-823细胞24、48、72小时后,细胞存活率显著降低,且与时间和剂量呈依赖关系。浓度为10%、15%、20%的含药血清作用于BGC-823细胞24小时后,G2期细胞增多,S期细胞减少。加药组BGC-823细胞凋亡率分别为(13.27±0.88)%、(19.97±0.60)%和(33.09±0.58)%,与空白对照组相同浓度空白血清细胞凋亡率(0.34±0.02)%、(0.19±0.04)%和(0.18±0.03)%比较,差异均有统计学意义(P<0.01)。结论:加味六君子汤含药血清对BGC-823细胞生长有明显抑制作用,并可诱导BGC-823细胞凋亡。Objective：To explore the effect of Jia-wei-liu-jun-zi decoction on proliferation and apoptosis of human gastric carcinoma cell line BGC-823 in vitro. Methods： BGC-823 cell line was cultured with 10% , 15% ,20% ,30% , and 40% of Jia-wei-liu-jun-zi decoction in serum,and its proliferation and apoptosis was detected by MMT and flow cytometric,respectively. Results：The survival of BGC-823 cells was decreased after 24,48,72h after culturing with different contents of Jia- wei-liu-jun-zi decoction ,and it was correlated with tile culturing time and contents. After 24h culturing with 10% , 15% , and 20% Jia-wei-liu-jun-zi decoction,the G2 time cell was increased but the S time cell was reduced ; the apoptosis rate of the 3 groups was （13.27±0.88）%,（19.97 ±0.60）%,（33.09±0.58）% as compared with （0.34±0.02）%,（0.19±0.04% ） , and （ 0.18 ±0. 03 ） %, respectively, with a significant difference （ P 〈0.01 ）. Conclusion ： Jia-wei-liu-jun-zi decoction can inhibit the growth of and may induce the apoptosis of BGC-823 cells. Key words gastric cancer BGC-823 Jia-wei-liu-jun-zi decoction serum medicated apoptosis proliferation

关 键 词：胃癌 BGC-823细胞 加味六君子汤 含药血清 细胞凋亡 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]