大麻性别相关AFLP分子标记筛选  

Screening of the AFLP markers linked to the sex locus of Cannabis sativa

作　　者：吕佳淑[1] 赵立宁[1] 臧巩固[1] 程超华[1] 唐晴[1] 李育君[1] 杨明[2] 郭鸿彦[2] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205 [2]云南省农业科学院经济作物研究所,云南昆明650205

出　　处：《湖南农业大学学报（自然科学版）》2010年第2期123-127,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家科技支撑计划项目(2006BAK09B03);农业部行业计划项目(nyhyzx07-018-05);国际科技合作与交流项目(2008DFR)

摘　　要：利用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)分子标记,用8个EcoRⅠ-NNN和8个MseⅠ-NNN组成64对引物,对大麻10个品种混合组成的雌性、雄性植株基因池进行筛选,并将筛选到的引物在此10个品种中进行验证,引物E-ACT/M-CTA在10个品种的雄株中均扩增出1条特异条带,雌株中均没有此带,对此引物扩增得到的特异性条带回收、克隆、测序,获得1条大小为348bp的雄性特异性条带,得到的序列进行GenBank序列比对,数据库中没有与此特异条带同源的序列.该条特异条带可作为分子遗传标记用于大麻早期性别鉴定的参考.AFLP（amplified fragment length polymorphism）was applied to identify markers associated with Cannabis sativa L.sex type. Sixty four pairs of primers of the 8 EcoR I -NNN and 8 Mse I -NNN combination were used to amplify the mixed male and female DNA pools of ten varieties. The primer E-ACT/M-CTA amplified an apparent fragment that was only existed in male pool and in all male individuals of the ten varieties. The male-specific fragment was 348 bp after recovering, cloning and sequencing. None of its homologous sequence was found in GenBank. Therefore, the male-specific fragment can be used as linkage marker in sex identification of Cannabis sativa.

关 键 词：大麻 扩增片段长度多态性 性别鉴定 

分 类 号：S563.303.2[农业科学—作物学]