番茄斑萎病毒属病毒SRNA序列比对  

The alignment of S RNA sequences in Tospovirus

作　　者：徐小刚[1] 刘雅婷[1] 李永忠[2] 何婷婷[1] 韩毅[1] 孙文涛[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学烟草学院,云南昆明650201

出　　处：《湖南农业大学学报（自然科学版）》2010年第2期142-146,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家"973"计划项目(2006CB100204);云南省自然科学基金项目(2005C0036M;2007C0036M);云南省教育厅项目(5Y0171B)

摘　　要：从生物信息数据库NCBI中搜索并下载最新有关番茄萎斑病毒属Tospovirus各种间SRNA片段全长序列以及该片段上NSs、N的核酸和蛋白质序列着手,运用DNAstar和DNAMAN以及NPSA等生物信息软件对其进行比对分析,结果发现,以NSs蛋白序列建立的系统进化树显示CCSV和TZSV之间的同源性为85.8%,IYSV和TYRV之间的同源性达90%,MYSV和PSMV间的同源性为97.5%;通过N蛋白序列分析显示,Tospovirus属中有7组同源性较高的种;在Tospovirus属病毒中非极性氨基酸含量最高,极性酸性氨基酸最少,比较TSWV中抗性破坏RB株系与普通株系在NSs、N核酸、蛋白质序列、氨基酸含量和蛋白质二级结构之间的差异,发现差异不显著.The nucleic acid and protein sequences on NSs, N and S RNA full-length sequences of Tospovirus genus were downloaded from NCBI database, and they were aligned by bio-informatics software DNAstar, DNAMAN and NPSA. It was demonstrated that homology between CCSV and TZSV was 85.8% on homologous phylogenetie tree on NSs protein sequences, homology between IYSV and TYRV reached 90%, homology between MYSV and PSMV was up to 97.5%. There are 7 groups of high homology with analysing the N protein sequences. Tospovirus is rich in non-polar amino acids but polar-acid amino acid is the lowest. At the same time, by alignment TSWV strains between resistance-breaking strain and wild strains on NSs, N nucleic acid and protein sequences, amino acids and the protein secondary structure with no significant differences found.

关 键 词：番茄斑萎病毒属 核壳体序列 S RNA上的非结构蛋白序列 抗性破坏 

分 类 号：S432.41[农业科学—植物病理学]