烟草青枯病菌的田间快速检测  

Rapid detection of tobacco bacterial wilt in the field

作　　者：杜桂萍[1] 周建国 邓金奇[1] 罗宽[1] 刘丹[1] 蔡勇[1] 肖启明[1] 

机构地区：[1]湖南农业大学生物安全科学技术学院,湖南长沙410128 [2]中国大拇指集团湖南大鼎置业有限公司,湖南长沙410002

出　　处：《湖南农业大学学报（自然科学版）》2010年第2期192-195,202,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省烟草专卖局重点项目(08-11Aa03)

摘　　要：用平板菌落计数法确定7种抗菌素对烟草青枯菌生长和抑制杂菌的最高浓度,通过正交试验确定药剂的最佳组合:安必仙9.996×10-5μg/mL、氧氟沙星9.990×10-7μg/mL、罗红霉素2.998×10-6μg/mL、头孢拉定3.000×10-6μg/mL、乙酰螺旋霉素1.998×10-5μg/mL、克拉霉素2.999×10-5μg/mL、阿奇霉素1.999×10-6μg/mL.利用以上最佳浓度配比的选择培养基检测湖南湘西、永州、郴州烟田土样中青枯菌,结果表明:湘西土样中青枯菌含量较大,永州次之,郴州相对较少,与青枯病发生的严重程度呈正相关,其中加青枯菌拮抗菌肥土壤中的含菌量明显少于未加菌肥土壤.The highest concentration of seven antibiotics on the growth of tobacco bacterial wilt, the inhabiting fungus and competitive bacterial were chosen with plate culture count. The results showed that ampicillin capsule was 9.996×10^-5μg/mL, ofloxacin tablets 9.990×10^- 7 μg/mL, roxithromycin tablets 2.998×10^-6 μg/mL, cefradine capsules 3.000×10^- 6 μg/mL, acetylspiramycin tablets 1.998×10^- 5μg/mL, clarithromycin dispersible tablets 2.999×10^-5 μg/mL, and azithromycin capsule was 1.999×10^-6 μg/mL. The selected medium of the optimal concentration ratio of seven antibiotics to detect tobacco bacterial wilt in the soil from different areas was used, and it was found that the number of tobacco bacterial wilt in soil samples in Xiangxi was larger than in Yongzhou, and relatively smaller in Chenzhou. There was a positive correlation between the disease index and the number of bacterial wilt, and the tobacco bacteria in adding antagonistic bacterial fertilizer soil were less than in common soil. The method can be used to detect tobacco bacterial wilt quickly and to predict the occurrence of tobacco wilt as well.

关 键 词：烟草青枯病 抗菌素 选择培养基 检测 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]