2种植物多糖对PRRSV灭活苗免疫猪抗体及T淋巴细胞亚群的影响  

Effects of two polysaccharides on T subpopulations and antibody in the immune response to PRRSV killed virus vaccine

作　　者：张红英[1,2] 崔保安[1,2] 王学兵[1,2] 赵现敏[1] 邱妍[3] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省动物性食品安全重点实验室,河南郑州450002 [3]河南科技大学动物科技学院,河南洛阳471004

出　　处：《湖南农业大学学报（自然科学版）》2010年第2期210-214,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：教育部博士点专项科研基金项目(20070466001);河南省基础与前沿技术研究计划项目(082300453207)

摘　　要：将24头35日龄断奶仔猪随机分为6组,2种多糖(山药多糖和黄芪多糖)分别以高、低2个剂量(山药多糖剂量17.10、8.55mg/kg,黄芪多糖剂量为11.50、5.75mg/kg)和猪PRRSV灭活苗同时注射,于免疫后不同时间点采血,监测PRRSV抗体水平和外周血中T淋巴细胞亚群的变化.结果表明,2种多糖均能增强免疫猪的体液免疫,其中以高剂量的黄芪多糖效果较好;2种多糖均能促进猪CD3+细胞的增殖,其中以山药多糖效果较好,提示2种多糖对PRRSV灭活苗免疫效果均有较好的增强作用.In order to study the effects of two polysaccharides on specific-humoral immunity and T subpopulations in piglets pheripheral blood in the immune response to PRRSV killed virus vaccine, 24 35-day-old piglets were randomly assigned into equal six groups and five groups were vaccinated with PRRSV killed virus vaccine. At the same time, the piglets in groups 1 to 4 were intramuscularly injected with Chinese yam polysaccharide（CYPS） at 17.10, 8.55 mg/kg, and astragalus polysaccharide（APS） at 11.50, 5.75 mg/kg, while group 5 with saline, once a day for three successive days, group 6 only injected with saline. On different time after vaccination, pheripheral blood were sampled for detection of serum antibody titer to PRRSV by ELISA and T lymphocytes subpopulations by cytofluorometry. The results showed that both polysaccharides could enhance the antibody titers, APS at high dosage was better than others and the two polysaccharides can improve the proportion ofCD3＋ and CD8＋ lymphocytes in the piglets pheripheral blood, CYPS was better than APS. These results indicated that both polysaccharides had significant immune enhancement effect on immunity of PRRSV killed virus vaccine in piglets.

关 键 词：山药多糖 黄芪多糖 猪繁殖与呼吸综合征病毒灭活苗 抗体 T淋巴细胞亚群 仔猪 

分 类 号：S853.74[农业科学—临床兽医学]