超声法提取骆驼蒿总生物碱的工艺研究  

Ultrasonic Wave Aided Extraction of Total Alkaloids from Peganum nigellastrum

作　　者：陈蕾[1] 于娟[1] 李晓蕙[1] 郭蕊[2] 王俊儒[1,2] 

机构地区：[1]西北农林科技大学理学院,陕西杨陵712100 [2]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300

出　　处：《西北林学院学报》2010年第3期158-162,共5页Journal of Northwest Forestry University

基　　金：2005年教育部新世纪优秀人才支持计划(NCET-05-0855);新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题(BR0805)

摘　　要：为优化骆驼蒿地上部分总生物碱的提取工艺,在单因素试验的基础上,选取乙醇浓度、提取温度、提取时间3个因素进行正交试验。结果表明:超声提取的影响因素排序为乙醇浓度>提取温度>提取时间;最佳提取条件为10倍量的80%乙醇提取3次,每次50 min,提取温度50℃,总生物碱提取量为1.960 mg.g-1。To optimize the technology of extracting total alkaloids from the above group of Peganum nigellastrum,orthogonal experiment was carried out with the factors of ethanol concentration,temperature and time on the basis of single-factor experiment.The results showed that influences of the factors were in the order of ethanol concentrationtemperaturetime.The optimal extraction conditions were the sample powder was extracted 3 times with 80% ethanol under the temperature of 50 ℃ for 50 min with a solid-liquid ratio of（w∶v）1∶10.Under this condition,the content of the alkaloids from P.nigellastrum was 1.960 mg·g-1.The extraction technology is simple and feasible.It provides a theoretical basis for extracting the total alkaloids of P.nigellastrum.

关 键 词：骆驼蒿 超声提取 总生物碱 正交试验 

分 类 号：S789.4[农业科学—林学] R284.2[医药卫生—中药学]