LBM2eHBc+融合基因植物表达载体构建及对番茄的转化研究  

Studies on Construction of Plant Expression Vector of Recombinant LBM2eHBc+ Gene and Tomato Transformation

作　　者：张国广[1,2] 张辉煌[1] 董美[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学和肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]漳州师范学院生物科学与技术系,福建漳州363000

出　　处：《漳州师范学院学报（自然科学版）》2010年第1期96-100,共5页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：福建省科技厅重大项目子课题(2006NZ0003-2);福建省教育厅A类科技项目(JA09168)

摘　　要：禽流感病毒一直是家禽和家畜健康养殖的巨大威胁,甚至威胁着人类的健康,人们也一直在研究各种形式的疫苗来应对禽流感病毒的威胁.番茄作为生物反应器应用于动物疫苗生产具有独特的优点,本研究优化了番茄子叶的再生体系,同时构建了LBM2eHBc+融合基因的植物表达载体并对番茄进行了遗传转化.结果表明在玉米素(Zt)浓度为0.5 mg/L时番茄子叶外植体的芽再生率达到93.33%;测序结果证明植物表达载体pBI121-LBM2eHBc+构建成功;利用农杆菌介导的转化方法获得再生抗性苗34株,经PCR检测,阳性率为75.0%,本研究结果为进一步开发禽流感口服疫苗奠定了基础.Avian influenza virus（AIV） infect almost all wild bird and domestic poultries and then decimate poultries,even the threat of AIV overhangs human.It also has been researching a variety of vaccines to combat the AIV threat.Tomato as a bioreactor used in animal vaccine production has a unique advantage.This study optimized the regeneration system of tomato cotyledon,while construct LBM2eHBc ＋ gene plant expression vector,and then carried out the genetic transformation of tomato.The results show that the bud regeneration rates of tomato reach the highest 93.33% when MS medium supplemented 0.5 mg/L Zt＋ 1 mg/L IAA;sequence results show that plant expression vector pBI121-LBM2eHBc ＋ constructed successfully.A total of 34 putative transgenic tomato plants were obtained by using of Agrobacterium-mediated transformation method,and the positive rate was 75.0% by PCR detection.The results laid a foundation for the further development of oral AIV vaccines.

关 键 词：禽流感病毒 LTB M2eHBc+ 转基因番茄 

分 类 号：Q943.2[生物学—植物学]