基于线粒体DNA全序列11个蛋白编码基因拼接序列的鳞翅目昆虫系统发育研究  

Phylogenetic Analysis within Lepidoptera Based on the Concatenated 11 Protein-coding Gene Sequences of Mitochondrial Genomes

作　　者：李青青[1] 段焰青[2] 李地艳[1,3] 李加敏[1] 刘晓飞[4] 徐怀亮[3] 李佛琳[5] 周汝敏[1] 曹能[1] 叶辉[4] 

机构地区：[1]云南师范大学生命科学学院,云南昆明650092 [2]红云红河烟草(集团)有限责任公司技术中心,云南昆明650202 [3]四川农业大学动物科技学院,四川雅安625014 [4]云南大学生命科学学院,云南昆明650091 [5]云南农业大学烟草学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2010年第2期213-218,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省自然科学基金项目(2006C0027Q)

摘　　要：目前已测定线粒体DNA全序列的鳞翅目昆虫有12种。由于高变异性和强核苷酸组成偏好性,去除了两个蛋白基因和两个物种,最终以10个鳞翅目物种的11个蛋白编码基因拼接序列对鳞翅目分子系统发育关系进行了研究。与前人的结果一致,基于11个蛋白编码基因拼接序列数据所构的最大似然树支持鳞翅目,鳞翅目内各总科和各科均为单系群。与MINET[1]基于形态学数据的结果相同,各总科的系统发育关系为{卷蛾总科+[螟蛾总科+(尺蛾总科+蚕蛾总科)]}。蚕蛾总科内各科系统发育关系类似于REGIER等[2]的结论。In this study, data set available for Lepidoptera so far, consisting of 12 complete （or almost complete） sequences of mitochondrial genomes, have been assembled. Concatenated sequences of 11 protein coding genes of 10 species were used to construct the phylogenetic relationships among Lepidoptera after removal of the high variation and extreme nucleotide composition bias. Based on these coding genes, results clearly confirmed that Lepidoptera was monophyletic by using maximum likelihood methods. Superfamilies and families were recovered, but not always strongly. Analysis of the concatenated 11 protein coding sequences by likelihood upholds the following relationships： {Tortricidea ＋ [ Pyraloidea ＋ （ Geometroidea ＋ Bombycoidea） ] }. The result wholly accords with the widely accepted phylogenetic hypotheses proposed by MINET based solely on morphological information. Within the Bombycoidea, our result reaches a similar conclusion with REGIER et al.

关 键 词：鳞翅目 系统发育 线粒体DNA全序列 

分 类 号：Q963[生物学—昆虫学]