基于HPLC法的丹参中丹参酮ⅡA的超声提取工艺优化研究  

Study on Optimization of Extraction Technology of Tanshinone IIA from Salvia miltiorrhiza Buhge with Ultrasonic Extraction Method Basea on HPLC

作　　者：刘常金[1] 师慧敏[1] 张治州[2] 张琼引[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457 [2]哈尔滨工业大学(威海)海洋学院分子营养与食品安全研究室,山东威海264209

出　　处：《安徽农业科学》2010年第8期4058-4059,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]确定超声波法提取丹参(Salvia miltiorrhiza Bunge)中丹参酮IIA的最佳提取工艺条件。[方法]以丹参药材为原料,在单因子试验基础上,采用正交试验对丹参酮IIA的超声波辅助提取工艺进行了优化,并用HPLC法测定了提取物中丹参酮IIA的含量。[结果]优化的提取条件为:在室温下,提取剂乙醇体积分数85%,提取时间30min,料液比(M:V)1:10,提取次数4次。在优化提取条件下,提取液中丹参酮IIA的含量可达到0.388%。[结论]用超声波法提取丹参酮IIA快速、高效、方便、节能,有利于规模化生产。[Objective]The aim was to optimize the extraction condition of tanshinone IIA from Salvia miltiorrhiza Buhge with ultrasonic extraction method.[Method] On the basis of single factor experiment,an optimum extracting technology of tanshinone IIA from S.miltiorrhiza was investigated through the orthogonal experiment under the ultrasonic wave assistance conditions,then the content of tanshinone IIA in extract was analyzed with HPLC method.[Result]The optimum extraction conditions were as follows：at room temperature,ethanol volume fraction 85%,extraction time 30 min,solid-liquid ratio 1∶10,extrating times 4.Under the above conditions,the content of tanshinone IIA in extract was 0.388%.[Conclusion]Extracting tanshinoneIIA with ultrasonic extraction method has characteristics of rapid,efficient,convenient and low energy consumption.It is benefit for producing tanshinoneIIA in large scale.

关 键 词：丹参 丹参酮ⅡA 超声提取法 高效液相色谱 

分 类 号：TQ461[化学工程—制药化工]