肺结核患者强化治疗前后血清差异蛋白质质谱分析  

Analysis of the serum differential proteins from patients with pulmonary tuberculosis before and after intensification therapy

作　　者：尹小毛[1] 刘志辉[1] 刘玉美[1] 罗强生[1] 谭守勇[1] 刘健雄[1] 

机构地区：[1]广州市胸科医院,广东广州510095

出　　处：《临床肺科杂志》2010年第5期697-698,共2页Journal of Clinical Pulmonary Medicine

基　　金：广州市科技计划项目(2006J1-C0211)

摘　　要：目的研究肺结核患者强化治疗前后血清蛋白质谱的变化。方法利用弱阳离子(WCX)液体蛋白质芯片和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术,对16例肺结核患者强化治疗前后的血清蛋白质谱进行比较分析。结果强化治疗前后有22种蛋白质的相对含量有明显差异,11种蛋白质在强化治疗后相对含量明显升高(P<0.05),另外11种蛋白质在强化治疗后相对含量明显降低(P<0.05)。结论肺结核患者强化治疗前后血清蛋白质谱存在明显差异。Objective To study the change of serum protein spectrum from patients with pulmonary tuberculosis before and after intensification therapy. Methods WCX fluid protein chip and matrix assisted laser desorption ionization/time of flight mass spectrometry were used to analyze the serum proteome of 16 patients with pulmonary tuberculosis before and after intensification therapy. Results After intensification therapy, relative quantity of 2：2 proteins was changed. 11 of 22 proteins were up-regulated and others were down-regulated（ P 〈 0. 05 ）. Conclusion Serum protein spectrum from patients with pulmonary tuberculosis is changed before and after intensification thera- py.

关 键 词：肺结核 基质辅助激光解吸电离质量光谱法 蛋白质组学 

分 类 号：R521[医药卫生—内科学]