啶菌噁唑对番茄灰霉病菌的抑菌作用研究  

Studies on antifungal activity of fungicide 5-(4-chloro phenyl)-2,3-dimethyl-3-(pyridine-3)-oxazoline aganist Botrytis cinerea

作　　者：陈凤平[1] 韩平[2] 张真真[1] 刘君丽[3] 刘西莉[1] 

机构地区：[1]中国农业大学农学与生物技术学院植物病理学系,北京100193 [2]北京农产品质量检测与农田环境监测技术研究中心,北京100097 [3]沈阳化工研究院,沈阳110021

出　　处：《农药学学报》2010年第1期42-48,共7页Chinese Journal of Pesticide Science

基　　金：2007年教育部新世纪优秀人才支持计划项目(NCET-07-0813);"十一五"国家科技支撑计划项目(2006BAD08A03)

摘　　要：采用生物测定方法研究了啶菌噁唑对番茄灰霉病菌Botrytis cinerea不同发育阶段的影响。结果表明:其对菌落扩展、芽管伸长、分生孢子产生和菌核形成均具有显著的抑制活性,其中啶菌唑对P9-菌株菌丝生长和芽管伸长的EC50值分别为0.193和0.154μg/mL,0.5μg/mL的啶菌噁唑对菌丝生长和芽管伸长的抑制率大于77%,对分生孢子和菌核萌发的抑制率分别低于10%和40%,表明药剂对分生孢子萌发无明显抑制作用。紫外吸收分析显示,啶菌噁唑可破坏细胞的膜结构,促使菌体核酸和蛋白外渗。离体叶片毒力测定结果表明,120μg/mL的啶菌噁唑可有效抑制番茄灰霉病病斑扩展,抑制率达83.74%。Effect of 5- （ 4-chlorophenyl ） -2, 3-dimethyl-3- （ pyridine-3 ） -oxazoline （ SYP-Z048 ） on different developmental stages of Botrytis cinerea was investigated by bioassay. The results showed that SYP-Z408 had significant inhibitory activity on colony growth, germ tube elongation, conidial and sclerotial production of B. cinerea. The EC50 value of SYP-Z048 against colony growth and germ tube elongation of strain P-9 was 0. 193 and 0. 059 μg/mL, respectively. SYP-Z048 can inhibit colony growth and germtube elongation of B. cinerea by more than 77% at the concentration of 0.5 μg/mL. However, it can only inhibit conidial and sclerotial germination by less than 10% and 40% at the same concentration, respectively. This result suggested that SYP-Z048 had little effect on conidial germination. The analysis of ultraviolet absorption revealed that SYP-Z048 could disrupt the cellular membrane structure, and caused the leakage of intracellular nucleic acid and protein from B. cinerea. The lesion extension of B. cinerea on tomato leaves could be inhibited effectively by 120 μg/mL of SYP-Z048, and the inhibitory rate was 83.74%.

关 键 词：啶菌噁唑 番茄灰霉病菌 发育阶段 抑菌作用 

分 类 号：S481.1[农业科学—农药学] S482.2[农业科学—植物保护]