小桐子离体培养的初步研究  

Preliminary Study of Jatropha curcas's Tissue culture in Vitro

作　　者：王小青[1,2] 陈雄庭[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海南海口571101 [2]海南大学农学院,海南海口570228

出　　处：《中国园艺文摘》2010年第4期20-23,共4页Chinese Horticulture Abstracts

摘　　要：分别以小桐子的叶柄和叶片为外植体,进行不同的BA和NAA浓度配比试验。结果发现:小桐子易于诱导愈伤组织。其中MS+6-BA1.0mg/L+NAA0.05mg/L效果最佳。最佳的生根配方为1/2MS+IBA0.2mg/L。用白糖和自来水代替对应的材料对试验结果影响不显著。Leaves were used as explants. Callus induction and plant regeneration were studied on MS medium containing different concentrations of 6-BA and NAA. The results showed that the callus ofJatropha curcas was easy to induce. The effect of medium MS＋6-BA1.0mg/L＋NAA0.05mg/L was the best among the all. The medium 1/2MS with 0.2mg/L IBA was best for the growth of adventitious roots. The edible sugar and tap water can be used to replace the sucrose and distilled water.

关 键 词：小桐子 叶片 离体培养 

分 类 号：S[农业科学]