6种冬青科苦丁茶总三萜类物质含量的比较研究  

Comparative Study on Contents of Total Triterpenoid of 6 Kudingcha Species in Aquifoliaceae

作　　者：李宏杨[1,2] 刘飞[3] 张凤琴[3] 李小龙[3] 刘国民[1,2] 

机构地区：[1]海南大学热带生物资源教育部国家重点实验室,海南海口570228 [2]海南大学苦丁茶研究所,海南海口570228 [3]湖南工业大学绿色包装与生物纳米技术应用湖南省重点实验室,湖南株洲412007

出　　处：《热带生物学报》2010年第1期17-20,共4页Journal of Tropical Biology

基　　金：国家科技支撑计划项目子课题(子课题编号:2007BAD76B05-02)

摘　　要：用φ=5%的香草醛-冰醋酸、高氯酸显色,以熊果酸纯品为对照,在波长548nm处用分光光度法测定了6种冬青科苦丁茶功能叶和4种市售苦丁茶冬青嫩芽产品中的总三萜含量。结果显示:大叶冬青功能叶中的总三萜类物质含量最高,市售苦丁茶冬青的嫩芽产品中其总三萜类物质含量最低。6种供试的冬青科苦丁茶样品中总三萜类物质的质量分数依次排序为:大叶冬青(4.209%)>枸骨(3.866%)>霍山冬青(3.064%)>华中枸骨(2.775%)>苦丁茶冬青(2.74%)>五棱冬青(2.648%)。5% vanillin aceturn and perchloric acid were used as the color developer, and the pure ursolic acid was used as the reference, the contents of total triterpenoid in adult leaves of 6 Kudingeha species in Aquifoliaceae and 4 kind marketed shoots products of llex kudingcha C. J. Tseng were determined by spectrophotometry at 548 nm. The results showed that the adult leaves of L latifolia Thunb. had the highest total triterpenoid, and the marketed shoots products of L kudingcha C. J. Tseng had the lowest total triterpenoid on average. The contents of total triterpenoid in 6 Kudingcha species in Aquifoliaceae for test samples were sorted as follows ： L latifolia Thunh. （4.209%）〉L cornuta Lihdl. （3.866%）〉 L huoshanesis Y. H. He （3.064）〉 L centrochinesis X. Y. Hu （2. 775% ） 〉 L kudingcha C. J. Tseng （2.74%） 〉 L pentagona S. K. Chen, Y. X. Feng et C. F. Liang （2.648%）.

关 键 词：苦丁茶 总三萜 熊果酸 

分 类 号：Q949.754.6[生物学—植物学] O657.32[理学—分析化学]