塔形马蹄螺精子发生的超微结构研究  

Ultra-structure Study on Spermatogenesis of Trochus pyramis Born

作　　者：吴洪流[1] 蒲利云[1] 冯永勤[1] 董杨[1] 刘璐[1] 严俊贤[1] 

机构地区：[1]热带生物资源教育部重点实验室(海南大学),海南大学海洋学院,海南海口570228

出　　处：《热带生物学报》2010年第1期21-26,共6页Journal of Tropical Biology

基　　金：海南省教育厅自然科学资助项目(琼教高HJKJ200721);海南省自然科学基金项目(0306)

摘　　要：应用透射电镜显微镜技术观察塔形马蹄螺(Trochus pyramis Born)精子的发生过程。结果表明:塔形马蹄螺精子的发生历经精原细胞、初级精母细胞、次级精母细胞、精细胞和成熟精子5个阶段。精原细胞呈近椭圆形,染色质分布较均匀,线粒体较少;初级精母细胞较大,染色质凝聚成小块状,线粒体增多;次级精母细胞较初级精母细胞小,线粒体较多,部分线粒体发生融合,呈扁囊状。精细胞的分化可分为5期,主要特点为:Ⅰ期,一些高电子致密颗粒参与顶体的形成;Ⅱ期,核形态由椭圆形到近圆形,再逐渐拉长为长圆柱状;Ⅲ期,核染色质以颗粒状形式凝集成较大的团块;Ⅳ期,线粒体逐步融合、体积增大和嵴发达;Ⅴ期,中心体移动及鞭毛形成,核内染色质凝聚成高电子密度均质化,胞质逐渐减少。成熟精子为原生型,由头部、中段和尾部(鞭毛)3部分组成。Transmission electron microscopy was used to observe the ultra-structure of spermatid of Trochus pyramis Born at different stages. The results indicated that the spermatogenesis of Trochus pyramis Born included following five stages： spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and spermatozoon. The shape of spermatogonium was ellipsoidal and there were few mitochondria, the distribution of chromatin uniform; the shape of primary spermatocyt was much bigger, the chromatin agglomerated as pieces and there were more mitochondria; Compared with primary spermatoeyte, secondary spermatocyte was smaller and there were more mitochondria, and some mitochondria fused and taken on cisterna shape. Spermiogenesis could be divided into five stages, the characteristics of the first stage： electron dense granules participated in the formation of acrosome; that of the second stage： the nucleus presented ellipsoidal shape or spherical shape, and become long cylinder gradually; that of the third stage： the chromatin agglomerated as larger pieces; that of the fourth stage ~ the mitochondria fused gradually, the volume improved and cristae developed ; that of the fifth stage ： centrosome moved and flagellar formed, the chromatin agglomerated further and become higher electron-densityhomegeneous, cytoplasm diminished. Mature spermatozoon was primitive type and composed of head, middle piece and tail.

关 键 词：塔形马蹄螺 精子发生 超微结构 

分 类 号：Q959.212[生物学—动物学]