流动注射化学发光法测定痕量的铅  

Chemiluminescence flow injection analysis of lead in water and human urine sample

作　　者：邱小香[1] 焦更生[1] 

机构地区：[1]渭南师范学院化学化工系,陕西渭南714000

出　　处：《西南民族大学学报（自然科学版）》2010年第2期257-261,共5页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：渭南师院自然科学基金资助项目(07YKZ005);渭南师院自然科学基金资助项目(09YKF006);陕西省教育厅2009年专项科研项目(09JK425)

摘　　要：铅会增强鲁米诺与高碘酸钾氧化反应所产生的化学发光,当铅的浓度为0.1-100.0 ng.mL-1时,化学发光强度的增加值与其浓度呈线性关系,化学发光试剂均固定在阴离子交换树脂上.当注入100μL水通过化学发光试剂柱,化学发光试剂被洗脱并产生化学发光,在铅存在时化学发光被增强.测定的检出限为30 pg·mL-1(3σ),线性相关系数r2=0.9993.在流速为2.0 mL·min-1时,完成一次铅的测定只需0.5分钟.相对标准偏差为3.0%.该法成功用于水样、人体尿液中铅的测定,且方法的选择性和灵敏度较高.A selective and sensitive CL flow injection method for the determination of lead （Ⅱ） Is described in this paper. The analytical reagents involved in CL reaction, including luminol and potassium periodate, are both immobilized on an anion exchange column. The CL signal produced by the reaction between luminol and potassium periodate, which are eluted from the column through water injection, is enhanced in the presence of lead （Ⅱ）. The increment of CL intensity is proportional to the concentration of lead （Ⅱ）, giving a calibration graph over the concentration from 0.1 to 100.0 ng·mL^-1 （r2= 0.9993） with the limit of detection of 30 pg.mL^-1（36）. At a flow rate of 2.0 mL.min^-1, a complete determination of lead （Ⅱ）, including sampling and washing, could be performed in 0.5 min with a relative standard deviation of less than 3.0%. The proposed procedure is applied successfully to the determination of lead （Ⅱ） in gasoline, water and human urine samples without any pre-treatment process. The proposed method offers reagentless procedures and remarkable stability for determining lead （Ⅱ）, and could be reused over 80h.

关 键 词：流通式传感器 化学发光 铅 水样 人体尿液 

分 类 号：O65[理学—分析化学]