抗丙烷脒番茄灰霉病菌渗透压敏感性及线粒体复合酶Ⅲ活性的变化  

Changes of osmolarity sensitivity and mitochondrial complex Ⅲ activity of propamidine-resistance isolates of Botrytis cinerea

作　　者：侯军[1,2] 高亚楠[1,2] 马志卿[1,2] 冯俊涛[1,2] 张兴[1,2] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心 [2]陕西省生物农药工程技术研究中心,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2010年第4期138-142,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30771442);教育部高等学校博士点基金项目(20070712003)

摘　　要：【目的】初步阐明番茄灰霉病菌抗丙烷脒的生理生化机制。【方法】以药剂连续选择获得的抗丙烷脒番茄灰霉病菌菌株和敏感菌株为研究材料,测定了抗药突变体的渗透压敏感性和线粒体复合酶Ⅲ活性的变化。【结果】敏感菌株对低渗透压和高渗透压均表现敏感,低抗菌株仅对低渗透压敏感,中抗菌株对低渗透压和高渗透压均不敏感。在离体条件下,丙烷脒对敏感和抗性菌株线粒体复合酶Ⅲ活性无明显影响;在活体条件下,丙烷脒处理对敏感菌株线粒体复合酶Ⅲ的活性有明显抑制作用,而对抗性菌株没有明显影响。【结论】渗透压敏感性下降和线粒体复合酶Ⅲ活性的改变,可能是番茄灰霉病菌对丙烷脒产生抗药性的主要原因。[Objective] Physiological and biochemical mechanism of Botrytis cinerea resistance to propamidine was clarified preliminarily. [Method] Propamidine-resistance strains of B. cinerea,which were used for testing osmolarity sensitivity and the activity of mitochondrial complex Ⅲ ,were obtained by continuous selection of propamidine. [Result] Sensitive strain was sensitive against low and high osmolarity. And,LR （low resistance） strain was sensitive against low osmolarity only. Besides, MR （moderate resistance） strain possessed no sensitivity against both low and high osmolarity. Propamidine had no effect on both sensitive strain and resistance-strains during the test of post-treatment on mitochondrial complex Ⅲ activity. However,during the pre-treatment activity test, propamidine possessed obvious inhibition on sensitive strains and had no significant effect on resistance-strains. [Conclusion] Descending of osmolarity sensitivity and changes of activitiy on mitochondrial complex Ⅲ might be the main factor for the resistance development of B. cinerea to propamidine.

关 键 词：番茄灰霉病菌 丙烷脒 渗透压 线粒体复合酶Ⅲ 

分 类 号：S436.412.13[农业科学—农业昆虫与害虫防治]