无菌培养条件下8个紫花苜蓿品种的苗期耐盐性研究  

Study on salt tolerance of 8 alfalfa varieties under sterile condition in seedling stage

作　　者：彭湖[1] 代锦[2] 呼天明[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2010年第4期176-182,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：农业部行业专项(nyhy2x07-22);国家科技支撑计划项目(2008BADB3B05);科技部国际合作项目(2006DFA33630)

摘　　要：【目的】探讨8个紫花苜蓿品种的耐盐性，为盐碱地改良与利用选择适宜的苜蓿品种。[方法】以8个紫花苜蓿品种种子为材料，在组织培养条件下，观测8个品种在不同浓度NaCI（MS培养基中NaCl浓度分别为0，50，85，120，170mmol／L）胁迫下的发芽率、单株鲜质量、根长、苗高以及游离脯氨酸含量的变化，并对其耐盐性进行综合评价。【结果】低浓度（50mmol／L）NaCl处理对紫花苜蓿的萌发和生长具有促进作用；随着NaCI浓度（85～170mmol／L）的升高，紫花苜蓿的发芽率和生长受到明显抑制，表现为相对发芽率、相对根长、相对苗高以及相对单株鲜质量明显下降，而游离脯氨酸含量明显升高。8个紫花苜蓿品种耐盐性的综合排序及其得分为“金皇后”（3．095）〉“巨人201”（2．467）〉“三得利”（2．379）〉“阿尔冈金”（2．369）〉“保定”（2．077）〉“中苜1号”（1．474）〉“敖汉”（0．885）〉“WL-232”（0．310）。【结论】“金皇后”是耐盐性最高的紫花苜蓿品种，适宜在土壤盐碱含量较高的地区种植；而耐盐性最差的是“敖汉”和“WL-232”，盐碱地应尽量避免选种这2个品种。[Objective] Salt tolerance of 8 alfalfa varieties was investigated in this study. [Method] The germination rate, the single fresh weight,the root length and the seedling height as well as the free proline content of 8 alfalfa varieties under different NaC1 concentrations（0,50,85,120,170 mmol/L in agar medi- um） were studied in vitro. Salt tolerance features were comprehensively assessed. [Result] The results indicated that lower concentration of NaCI（50 mmol/L）promoted germination and growth of alfalfa; However germination rate and growth of alfalfa were restrained prominently with the increase of NaCl contents （85,120,170 mmol/L）. The relative germination, the relative single fresh weight, the relative root length and the relative height decreased significantly with the increase of NaCl concentration, while the free pro- line content increased significantly. According to the results of integrated evaluation, the sort and score of the salt tolerance of seeds of 8 varieties were ＂God empress＂ （3. 095））＂Ameristand 201＂（2. 467））＂Sandity＂（2. 379））＂Algonquin＂（2. 369））＂Baoding＂（2. 077））＂Zhongmu No. 1＂（1. 474））＂Aohan＂（0. 885）） ＂WL-232＂（0. 310）. [Conclusion] ＂God empress＂ is the most salt-tolerant alfalfa variety,and is suitable forplanting in the high saline-alkali soil;While ＂Aohan＂ and ＂WL-232＂ are the least salt-tolerant alfalfa varieties, and we should avoid planting them in the high saline-alkali soil.

关 键 词：紫花苜蓿 组织培养 耐盐性 综合评价 

分 类 号：S551.7[农业科学—作物学]