我国HIV-1 CRF07_BC流行重组毒株Tat蛋白第一外显子CTL表位预测  

Prediction of CTL epitopes of Tat exon 1 region in HIV-1 CRF07_BC strains in China

作　　者：方志明[1] 邢辉[2] 孟哲峰[2] 洪坤学[2] 廖玲洁[2] 何翔[2] 吕建新[1] 邵一鸣 

机构地区：[1]温州医学院生命科学学院,325035 [2]中国疾病预防控制中心性病艾滋病预防控制中心传染病国家重点实验室,北京100050

出　　处：《中华微生物学和免疫学杂志》2010年第2期135-139,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目（30671847）;国家＂973＂重点基础研究发展计划（2005CB522903）;十一五重大传染病专项（2008ZX10001-004）

摘　　要：目的 通过测定和分析我国HIV-1 CRF07_BC毒株Tat蛋白第一外显子的基因序列预测其CTL表位的分布情况.方法 236份来自第3次全国HIV分子流行病学调查中感染CRF07_BC毒株的患者的血浆样本,应用巢式聚合酶链反应（nested-PeR）扩增tat基因第一外显子基因区并测序,使用BIMAS HLA Peptide Binding Predictions在线软件和统计学软件对CTL表位进行预测分析.结果 236份CRF07_BC毒株来自16个省份,主要为静脉吸毒传播（58.9%）,其次为性传播（25.0%）.236条CRF07_BC Tat蛋白第一外显子区共分布有12种CTL表位,主要分布于脯氨酸富集区、半胱氨酸富集区和疏水核心区,碱性区和谷氨酰胺富集区没有发现已知的CTL表位分布,这些表位主要受5种HLA基因型（A ＊ 2501、A ＊ 2902、B ＊ 15、B ＊ 5301和Cw ＊ 1203）以及6种HLA血清型（B53、B58、B57、A3、A68和Cw12）限制,其中5种表位的单氨基酸变异频率大于50%.结论 我国HIV-1 CRF07_BC毒株Tat蛋白第一外显子CTL表位分布于不同功能区,并且存在氨基酸变异.Objective To predict the CTL epitopes of Tat exon 1 region in HIV-1 CRF07_BC strains, which were prevailing in China. Methods Total of 236 plasma samples were from the 3rd National HIV Molecular Epidemic Survey （NMES3）. All the subjects were infected with HIV-1 CRF07_BC viruses. The tat exon 1 region was amplified by reverse transcription reaction and nested polymerase chain reaction （nested-PCR）, then the PCR products were sequenced. The distribution of CTL epitopes of this region were predicted by on-line software BIMAS HLA Peptide Binding Predictions and statistics software. Results To-tal of 236 CRF07_BC strains were from 16 provinces, mainly in intravenous drug asers（58.9%）and then sex（25.0%）. It was showed that there were 12 CTL epitopes of 236 Tat exon 1 region of CRF07_BC strains mainly located in proline-rich region, cysteine-rich region and core-region. Those epitopes were banded by 5 HLA presenting molecules in genotype（A ＊ 2501 ,A ＊ 2902, B ＊ 15,B ＊ 5301 and Cw ＊ 1203） and 6 HLA presenting molecules in serotype （B53, B58 ,B57 ,A3 ,A68 and Cw12）. The frequency of single amino acid substitution was more than 50% in 7 CTL epitopes. Conclusion The CTL epitopes in Tat exon 1 of CRF07 _BC strains were located in different functional regions, and there were some amino acid variations in them.

关 键 词：人免疫缺陷病毒 TAT蛋白 变异 表位 

分 类 号：R440[医药卫生—诊断学]