P7抑制bFGF诱导的3T3细胞增殖的作用机制  

Mechanism of inhibitory effect of P7 on 3T3 cell proliferation induced by basic fibroblast growth factor

作　　者：王聪[1,2] 林绍强[2,3] 李校堃[3] 吴晓萍[1,3] 

机构地区：[1]暨南大学组织移植与免疫实验中心,广东广州510632 [2]暨南大学医学院,广东广州510632 [3]温州医学院药学院,浙江温州325035

出　　处：《药学学报》2010年第3期314-317,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30973671);广东省自然科学基金资助项目(9151064001000031);温州市科技计划资助项目(Y20090244);生物化学与分子生物学广东省重点学科资助项目

摘　　要：研究从噬菌体随机七肽库中筛选得到的新型bFGF拮抗肽(P7)抑制bFGF诱导的Balb/c3T3细胞增殖的作用机制。采用倒置显微镜观察不同浓度P7对细胞形态的影响;流式细胞术分析P7对细胞周期的影响;Western blotting检测P7对bFGF刺激的Balb/c3T3细胞中MAPK通路的MEK、Erk1/2信号分子活化水平的影响。结果显示,在检测的浓度范围内,P7对Balb/c3T3细胞形态无显著影响;P7可改变bFGF刺激下Balb/c3T3的细胞周期,减少S期细胞比率,使细胞阻滞在G0/G1期;并以剂量依赖的方式下调bFGF激活的MEK、Erk1/2信号分子的磷酸化水平。P7可能通过阻滞细胞在G0/G1期,下调MAPK通路信号分子的活化水平,从而抑制bFGF诱导的Balb/c3T3细胞增殖。To investigate the mechanism of inhibitory effect of a novel bFGF antagonist peptide isolated from the phage display random heptapeptide library on cell proliferation induced by basic fibroblast growth factor. The effect of P7 on cell morphology was observed under an inverted microscope. Flow cytometry was applied to analyze the effect of P7 on cell cycle progress of bFGF-stimulated cells. The effect of P7 on bFGF-induced activation of MEK and Erk1/2 in MAPK pathway was detected by Western blotting. The results showed that no significant cell morphology change was observed in the range of detected concentrations of P7. Cell cycle analysis showed that P7 decreased S-phase cell population and arrested cell cycle at the G0/G1 phase of bFGF-stimulated cells. The results of MAP kinase activation assay indicated that P7 decreased bFGF-induced MEK and Erk1/2 phosphorylation in a dose-dependent manner. P7 inhibited proliferation of bFGF-stimulated Balb/c 3T3 cells possibly via cell cycle arrest at the G0/G1 phase and down-regulation of signal molecular activation in MAPK pathway.

关 键 词：BFGF 拮抗肽 细胞周期 MAPK通路 

分 类 号：R966[医药卫生—药理学]