眼针对肠易激综合征大鼠血清和结肠组织中P物质及血管活性肠肽含量的影响  

Effects of Eye-acupuncture Therapy on Serum and Colonic SP and VIP Contents in Rats with Irritable Bowel Syndrome

作　　者：王艳杰[1] 王德山[1] 关洪全[1] 王健[1] 柴纪严[1] 赵金茹[1] 韩晓伟[1] 

机构地区：[1]辽宁中医药大学基础医学院,沈阳110032

出　　处：《针刺研究》2010年第1期8-11,26,共5页Acupuncture Research

基　　金：国家重点基础研究发展计划("973"计划)资助项目(No.2007CB512702)

摘　　要：目的:观察眼针疗法对肠易激综合征(IBS)大鼠血清和结肠组织中P物质(SP)和血管活性肠肽(VIP)含量的影响,探讨眼针疗法对IBS治疗的作用机制。方法:40只雄性Wistar大鼠随机分为正常组、模型组、眼针组和匹维溴胺组,每组10只。采用慢性应激结合束缚方法建立IBS大鼠模型。眼针组取下焦区、大肠区、肝区、脾区进行针刺,治疗7 d。匹维溴胺组给予匹维溴胺灌胃7 d。HE染色检测结肠组织病理学变化;酶联免疫吸附法测定大鼠血清和结肠组织中SP和VIP含量的变化。结果:各组大鼠结肠组织病理学变化没有明显差异。模型组大鼠与正常组比较,血清和结肠组织中SP和VIP含量显著升高(P<0.01);眼针治疗组和匹维溴胺组与模型组比较,血清和结肠组织中SP和VIP含量显著降低(P<0.01)。结论:眼针疗法能够降低IBS模型大鼠血清和结肠组织中SP和VIP的含量,从而对IBS起到治疗作用。Objective To observe the effect of the eye-acupuncture therapy on serum and colonic substance P （SP） and vasoactive intestinal peptide （VlP） contents in rats with irritable bowel syndrome （IBS） so as to explore its underlying mechanism. Methods Forty male Wistar rats were equally randomized into control group, IBS model group, eye-acupuncture group and medication （Pinaverium bromide, 7.5 mg/kg, twice daily, intragastric administration） group. IBS model was established by giving the rat with chronic stress stimulation （cold-water swimming, tail clamping, electrical shock, etc. ） for 18 days. Eye-acupuncture of Xiajiao （Low Energizer） Area, Pi （Spleen） Area, Gan （Liver） Area and Dachang （Large Intestine） Area was given to the rat 20 min, twice daily for 7 d. Histopathological changes of the colon tissue were displayed by HE staining; and serum and colonic SP and VlP contents were detected by enzyme linked immunosorbent assay （ELISA）. Results No significant difference was found among 4 groups in the histopathological changes of the colon. In comparison with normal control group, both serum and colonic SP and VlP contents in model group increased significantly （P〈0.01）, while compared with model group, those in eyeacupuncture and medication groups lowered considerably （P〈0.0]）. Conclusion Eye-acupuncture can reduce serum and colonic SP and VlP contents in IBS rats, which may play a role in relieving IBS in eye-acupuncture clinic.

关 键 词：眼针 肠易激综合征 P物质 血管活性肠肽 

分 类 号：R245.329[医药卫生—针灸推拿学]