电针对偏头痛大鼠的皮层扩展性抑制以及对血浆降钙素基因相关肽、P物质含量的影响  

Effect of Electroacupuncture on Cortical Spreading Depression and Plasma CGRP and Substance P Contents in Migraine Rats

作　　者：石宏[1] 李江慧[1] 吉长福[1] 尚红艳[1] 邱恩超[2] 王京京[1] 景向红[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]解放军总医院神经内科,北京100853

出　　处：《针刺研究》2010年第1期17-21,共5页Acupuncture Research

基　　金：国家自然科学基金资助(30901932)

摘　　要：目的:观察针刺对偏头痛大鼠皮层扩展性抑制(CSD)电位以及体内降钙素基因相关肽(CGRP)及P物质(SP)含量的影响,探讨针刺治疗偏头痛的机制。方法:将SD大鼠随机分为正常组、模型组和电针组,每组10只。模型组在皮层顶叶局部给予3 mol/L KCl诱导CSD,在额叶放置记录电极;电针组在皮层施予3 mol/L KCl的同时电针双侧"阳陵泉""太冲"穴30 min。观察偏头痛大鼠在电针前后CSD的波幅变化,放射免疫法测定大鼠血浆中CGRP及SP含量的变化。结果:模型组给予KCl后,经过3 min左右诱导出CSD,表现为负向电位,幅度为(-25.13±1.23)mV,电针组经电针治疗可明显抑制CSD的波幅,平均幅度为(-19.19±1.53)mV,二者相比差异有统计学意义(P<0.01);电针抑制CSD波幅的作用可持续50 min;模型组大鼠的血浆中CGRP含量明显增高,与正常组相比差异有统计学意义(P<0.001),而经过电针治疗的偏头痛大鼠血浆中CGRP含量较模型组明显降低(P<0.05)。模型组血浆中的SP含量与正常组相比显著升高(P<0.01),经电针治疗后大鼠血浆中SP含量较模型组显著下降(P<0.001)。结论:电针"阳陵泉"和"太冲"可以显著抑制偏头痛大鼠CSD的波幅,降低大鼠血浆中的CGRP和SP含量,提示电针对偏头痛的治疗作用可能是通过调节血浆中的CGRP及SP含量实现的。Objective To observe the effect of electroacupuncture （EA） on cortical spreading depression （CSD） and contents of plasma calcitonin gene-related peptide （OGRP） and substance P （SP） in migraine rats. Methods Thirty male SD rats were equally randomized into control, model and EA groups. Migraine model was established by topical application of KOl （3 mol/L） immersed in a piece of filter paper to the cerebral cortex （parietal lobe, 6 mm posterior to the Bregma and 5 mm to the sagital fissure） after exposure of the skull （in reference to Michael＇ method）. KCl stimulation evoked CSD potentials （3 mm rostral to the Bregma, and 2 mm to the sagital fissure） were recorded by using a glass microelectrode. For rats of control group, filter paper containing 0.9 % NaCl was applied to the same parietal cortex area. EA （1 mA, 2 Hz/100 Hz） was applied to bilateral ＂Yanglingquan＂ （GB 34） and ＂Taichong＂ （LR 3） for 30 min. The contents of plasma CGRP and SP were assayed by radioimmunoassay. Results CSD was induced 3-5 min after application of KCI to the parietal lobe. The average amplitude of model group was （-25. 13± 1.23） mV, and that of EA group was （- 19. 19± 1.53） mV, displaying a significant reduction of OSD amplitude after EA（P〈0.01）. Qomparison among 3 groups showed that both plasma CGRP and SP contents in model group were significantly higher than those of control group （P〈0. 001, P〈0.01 ）, while compared with model group, plasma OGRP and SP levels in EA group decreased considerably （P〈0.05, P〈0. 001 ）, suggesting an inhibitory effect of EA on pain-producing substance.Conclusion EA of GB 34 and LR 3 can effectively suppress KCI provoked cortical spreading depression and plasma CGRP and SP levels in the rat, which may contribute to its effect in relieving migraine in clinic.

关 键 词：偏头痛 皮层扩展性抑制 电针 降钙素基因相关肽 P物质 

分 类 号：R245.97[医药卫生—针灸推拿学]