海兔素对人胃癌细胞SGC-7901增殖和凋亡的影响  

Effect of aplysin on the proliferation and apoptosis in human gastric cancer cell SGC-7901

作　　者：刘颖[1] 梁惠[2] 苏爱[1] 贺娟[3] 于红[4] 

机构地区：[1]青岛大学医学院细胞与分子生物学实验室,山东青岛266071 [2]青岛大学医学院营养研究所,山东青岛266021 [3]青岛大学医学院附属医院营养科,山东青岛266003 [4]青岛大学医学院病原生物学教研室,山东青岛266071

出　　处：《中国药理学通报》2010年第3期333-337,共5页Chinese Pharmacological Bulletin

基　　金：山东省科技厅科技攻关项目(No2006GG2302002);青岛市科技局科技计划资助项目(No08-2-1-5-nsh)

摘　　要：目的研究海兔素(Aplysin)对人胃癌SGC-7901细胞增殖和凋亡的影响。方法MTT法测定海兔素对胃癌SGC-7901细胞增殖的影响;倒置显微镜观察细胞生长状态的变化;HE染色检测细胞凋亡情况;RT-PCR法检测SGC-7901细胞中COX-2mRNA的表达。结果经60、120、240、480mg.L-1海兔素作用24、48h后,SGC-7901细胞的生长增殖均明显受到抑制,呈量效依赖性。延长作用时间,抑制作用差异无显著性(P>0.05);120、240mg·mL-1海兔素作用24h后,细胞生长状态明显下降;经120、240mg·mL-1海兔素处理18h后,细胞凋亡率分别为(15.0±2.12)%和(18.4±2.30)%,与对照组(1.4±0.55)%比较差异均有显著性(P<0.05);60、120、240mg·mL-1海兔素处理24h后,SGC-7901细胞COX2mRNA水平有下调趋势,但差异无显著性(P>0.05)。结论海兔素对人胃癌SGC-7901细胞具有抑制增殖和诱导凋亡的作用。Aim To investigate the effects of Aplysin on the inhibition of gastric cancer cell in vitro.Methods MTT assay was used to examine the inhibition of gastric cancer cell 1ine SGC-7901 by Aplysin in different concentrations and at different times.The morphologic changes and the apoptosis of SGC-7901 was observed by inverted microscope and Hematoxylin-Eosin（HE）staining.Reverse transcriptase polymerase chain reaction（RT-PCR）assay was used to detect the changes of COX-2 mRNA expressions.Results Aplysin could decrease the proliferation significantly in a dose-dependent manner in SGC-7901 cells.When treating SGC-7901 with Aplysin in concentration of 120,240 mg·L-1 for 24 h,the growth of the cell was obviously inhibited observing by inverted microscope.Aiso,when treating with the same concentration for 18 h,its chromatin became crimpled and breakdown,as well as cell shrinkage and apoptotic bodies formation when using HE staining.The apoptotic rates（%）of SGC-7901 was（15.0±2.12）%,（18.4±2.3）%,respectively,which was significantly higher than（1.4±0.55）% that in control group（P0.01）.60、120、240 mg·L-1 Aplysin could not effectively inhibited the mRNA expressions of COX-2（P0.05）.Conclusions Aplysin can inhibit the proliferation and induces apoptosis of SGC-7901 cells.

关 键 词：海兔素 萜 SGC-7901细胞 MTT 凋亡 RT-PCR 环氧合酶-2 

分 类 号：R282.77[医药卫生—中药学] R979.1[医药卫生—中医学]