人参皂苷Rg1对大鼠肠缺血/再灌注损伤的影响  

Effect of ginsenoside Rg1 on gut injury following intestinal ischemia reperfusion in rats

作　　者：李茜[1] 张彦敏[2] 关玥[1] 马会杰[1] 高璐[1] 张翼[1] 

机构地区：[1]河北医科大学基础医学院生理学教研室,河北石家庄050017 [2]石家庄第二医院神经内科,河北石家庄050000

出　　处：《中国药理学通报》2010年第3期358-361,共4页Chinese Pharmacological Bulletin

基　　金：河北省科技厅计划资助项目(No07276437)

摘　　要：目的观察人参皂苷Rg1(ginsenoside Rg1)对大鼠肠缺血/再灌注后肠组织损伤的影响,并探讨其机制。方法制备SD大鼠肠缺血/再灌注损伤模型。实验分为3组(n=10):假手术组、缺血/再灌注组(对照组)、人参皂苷Rg1干预组(治疗组)。比较各组大鼠肠黏膜肿瘤坏死因子α(TNF-α)水平、白细胞介素6(IL-6)水平、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性的变化;用Chiu氏评分法观察肠黏膜的损伤情况。结果与假手术组相比,对照组TNF-α、IL-6水平明显升高,MDA含量明显增高,SOD活性明显降低,小肠损伤评分明显升高;与对照组相比,治疗组TNF-α、IL-6水平明显降低,MDA含量明显降低,SOD活性明显升高,小肠损伤评分明显降低。结论人参皂苷Rg1对大鼠肠缺血/再灌注后的肠道有保护作用,该保护作用可能与减少TNF-α、IL-6、MDA含量,提高SOD活性有关。Aim To investigate the effect of ginsenoside Rg1 on gut injury following intestinal ischemia reperfusion in rats and the possible mechanism.Methods By using rat model of intestinal I/R injury,30 male SD rats were randomly divided into 3 groups（n=10 in each group）：sham-operation group,I/R group（control group）and ginsenoside Rg1 group（treatment group）.The contents of tumor necrosis factor α（TNF-α）,interleukin-6（IL-6）,malondialdehyde（MDA）,the activity of superoxide dismutase（SOD）in intestinal mucosa were measured respectively.Chiu＇s count was used to assess the changes in intestinal pathological morphology.Results TNF-α,IL-6,MDA contents and the intestinal injury score in control group were significantly increased compared to those in sham-operation group,while SOD contents in control group were significantly decreased compared to sham-operation group.Inversely,TNF-α,IL-6,MDA contents and the intestinal injury score in treatment group were significantly decreased compared to those in control group,while SOD contents in treatment group were significantly increased compared to control group.Conclusion Pretreatment with ginsenoside Rg1 has protective effect on intestinal ischemia-reperfusion injury in rats,which may be attributed to decreased contents of TNF-α,IL-6,MDA and increased levels of SOD.

关 键 词：人参皂苷RG1 小肠 缺血/再灌注 肿瘤坏死因子Α 白细胞介素6 丙二醛 超氧化物歧化酶 

分 类 号：R-332[医药卫生] R284.1