人参皂苷Rg1对细胞光老化模型中p53信号转导途径的影响  

Ginsenoside-Rg1 protects human dermal fibroblasts against 8-methoxypsoralen plus ultraviolet A-induced photoaging through p53-dependent signaling pathways

作　　者：高瑛瑛[1] 刘文丽[1] 周炳荣[1] 李威[1] 骆丹[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,江苏南京210029

出　　处：《中国药理学通报》2010年第3期383-387,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30873407)

摘　　要：目的观察人参皂苷Rg1对光老化p53信号转导途径中相关基因损伤及蛋白表达水平的影响。方法采用8-MOP/UVA(8-methoxypsoralen and subsequent ultraviolet A irradiation)联合处理人皮肤成纤维细胞建立光老化模型,用流式细胞周期分析、SA-β-半乳糖苷酶(senescence associatedβ-galactosidase)化学染色,免疫荧光及Western blot等方法检测人参皂苷Rg1对培养真皮成纤维细胞多项细胞衰老指标及p53信号途径中蛋白表达的影响。结果人参皂苷Rg1可明显抑制细胞和组织老化的指标表达(包括SA-β-Gal表达减少及细胞周期G1阻滞率降低);减少基因氧化应激损伤产物8-oxo-dG及老化相关蛋白p53,p21WAF-1及p16INK-4a的表达。结论人参皂苷Rg1可能通过缓解基因的氧化应激损伤,抑制相关信号转导,从而缓解细胞光老化进程。Aim To investigate the roles of p53-dependent signaling pathways in the process of ginsenoside Rg1 protection against 8-methoxypsoralen（8-MOP）and subsequent ultraviolet A（UVA）irradiation induced photoaging model in human dermal fibroblasts（HDFs）.Methods Photoaging model was established by 8-MOP/UVA in skin HDFs.Flow cytometry,enzyme cytochemistry,immunofluorescence and Western blot were employed.Results Pretreatment with ginsenoside Rg1 could significantly reduce the amount of UVA-generated 8-oxo-dG and relieve the photoaging representation.Compared with 8-MOP/UVA treatment group,pretreatment with Ginsenoside Rg1 could decrease the expression of SA β-galatosides（SA-β-Gal）,and down-regulate the level of senescence associated proteins（p16 and p21）.Conclusions Ginsenoside Rg1 has prominent dose-dependent antagonism on senescence of skin HDFs induced by 8-MOP/UVA,and its mechanism may be due to its antioxidation which reduces the production of photoproducts to protect telomere against abnormal shortening.

关 键 词：人参皂苷RG1 光老化 8-oxo-dG P53 p21WAF-1 p16INK-4a 

分 类 号：R284.1[医药卫生—中药学] R322.99[医药卫生—中医学]