一种小鼠高脂血症新模型的建立  

The establishment of a new models on experimental hyperlipidemia mice

作　　者：吴争荣[1] 马志刚[1] 董永喜[1] 黄金程 贺殿[1] 

机构地区：[1]兰州大学药学院,甘肃兰州730000

出　　处：《中国药理学通报》2010年第3期405-407,共3页Chinese Pharmacological Bulletin

基　　金：兰州市科技局基金资助项目(No市科技06-4-09)

摘　　要：目的建立高脂血症实验动物模型新型方法。方法30只小鼠随机分为空白对照组、阳性对照组及模型组。空白对照组喂饲标准饲料,阳性对照组喂饲高脂饲料;模型组饲以标准饲料和牛奶,30d后检测血清和肝脏胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及肝脏指数(LI),光镜观察肝脏病理变化。结果与空白组相比,模型组LI及血液和血清中TC、TG、LDL-C明显升高,而HDL-C明显降低。光镜下模型组肝脏均出现弥漫性肝细胞脂肪变性和炎症病灶。结论小鼠喂饲30d标准饲料和牛奶,可建立理想的高脂血症模型。Aim To establish an ideal hyperlipidemia animal model by a new way.Methods 30 mice were randomly divided into control group,positive control group and model group.The mice of control group were only fed with a standard diet.Those of positive control group were fed with high fat diet.Those of model group were fed with a standard diet and enough milk.After 30 days the TG,TC,HDL-C and LDL-C of serum and hepatic and LI were detected and pathological changes in the liver of mice were observed microscopically.Results Compared with the control group,the mice of model group developed hyperlipidemia with LI and the serum and the hepatic TG,TC,LDL-C elevated significantly,while HDL-C were significantly lower.The histopathological research showed hepatocellular macrovesicular steatosis and hepatitis in the model group.Conclusion An ideal model of hyperlipidemia is successfully established with standard diet and milk fed to mice for 30 days.

关 键 词：高脂血症 动物模型 小鼠 高脂饲料 牛奶 血清 肝脏 

分 类 号：R332[医药卫生—人体生理学] R322.47[医药卫生—基础医学]