CD14的基因型及血清水平与急性心肌梗死的关系  

Serum CD14 levels and the genotypes of CD14 gene in patients with acute myocardial infarction

作　　者：刘忠仁[1] 刘运广[1] 韦叶生[2] 潘兴寿[1] 黄照河[1] 蒙兰青[1] 

机构地区：[1]右江民族医学院附属医院心血管内科,广西百色533000 [2]右江民族医学院附属医院临床检验中心

出　　处：《中国老年学杂志》2010年第6期743-745,共3页Chinese Journal of Gerontology

基　　金：广西青年科学基金资助项目(0447060)

摘　　要：目的研究CD14基因启动子-159C/T、-260C/T多态性各等位基因及基因型在急性心肌梗死(AMI)患者中的分布频率,初步分析其基因型及血清水平与AMI的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,检测120例AMI患者及130例正常对照组CD14的基因多态性,同时采用酶联免疫吸附试验(ELISA)检测血清CD14水平。结果AMI组血清CD14水平显著高于对照组(P<0.01),CD14基因-159C/T多态性在AMI组和正常人群中的分布差异无显著性(P>0.05),而CD14基因-260C/T多态性在两组人群中的分布差异存在显著性(P<0.05),等位基因频率的相对风险分析发现,T等位基因携带者患AMI的风险是C等位基因的1.654倍(OR=1.654,95%CI:1.161～2.356),携带T等位基因的AMI患者血清CD14水平显著高于不携带者(P<0.05)。结论CD14基因启动子-260C/T多态性与AMI的发病具有相关性,其中T等位基因可能是AMI发病的遗传易感基因;携带T等位基因的个体可能通过促进CD14的高度表达进而增加了AMI的发病风险。Objective To study the relationship the allele frequencies and genotype distribution of CD14 gene promoter region-159C/T,-260C/T polymorphisms in Chinese patients with acute myocardial infarction （AMI）,and to analyze association between the serum levels and genotype of CD14 with AMI.Methods The polymorphisms of CD14 gene were analyzed by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） methods in 120 AMI patients and 130 healthy controls,and the serum level of CD14 was determined by enzyme-linked immunosorbent assay （ELISA）.Results The AMI group showed significantly higher serum levels of CD14 than control group（P0.01）,the distributions of CD14 gene-159C/T polymorphism was not different between AMI group and control group,but the CD14 gene-260C/T polymorphism was significantly different.The relative risk suffered from AMI of T allele was 1.654 times of the C allele（OR=1.654,95%CI：1.161～2.356）,the serum level of CD14 T allele carriers was significantly higher than that of no carriers（P0.05）.Conclusions CD14 gene promoter region-260C/T polymorphism is associated with AMI,and T allele may be a risk factor for AMI.In which the CD14 T allele carriers maybe increase risk by enhancing the CD14 expression in the pathogenesis of AMI.

关 键 词：急性心肌梗死 CD14 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病]