生长抑素对大鼠胰腺腺泡细胞内骨架蛋白F-actin分布和细胞外分泌功能的影响  

Effect of somatostatin on F-actin distribution and exocrine function in rat pancreatic acinar cells

作　　者：黄刚[1] 汤为学[2] 陶小红[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016 [2]重庆医科大学附属第一医院重庆市神经病学重点实验室,重庆400016

出　　处：《第三军医大学学报》2010年第7期672-676,共5页Journal of Third Military Medical University

摘　　要：目的研究生长抑素（somatostatin,SS）对八肽胆囊收缩素（cholecystokinin-octapeptide,CCK-8）刺激的大鼠胰腺腺泡细胞内骨架蛋白F-actin分布变化和细胞外分泌功能的影响。方法分离的19只SD大鼠胰腺腺泡细胞,设空白对照组、不同浓度CCK-8组（10^-12、10^-11、10^-10、10^-9、10^-8mol/L）、SS组（10^-7mol/L）、SS＋CCK-8组。SS与不同浓度的CCK-8共同孵育胰腺腺泡细胞30min,用淀粉酶、胰蛋白酶试剂盒检测胰腺腺泡细胞淀粉酶分泌率、胰蛋白酶活性,用激光共聚焦显微镜观察细胞内F-actin分布的变化。结果SS稳定了细胞内骨架蛋白结构,明显提高F-actin近细胞顶部/侧基底部的比值（P〈0.05）;SS与低浓度的CCK-8（10^-11-10^-10mol/L）共同孵育胰腺腺泡细胞时,CCK-8诱导的细胞淀粉酶分泌明显被抑制（P〈0.05）,SS与高浓度CCK-8（10^-9-10^-8mol/L）共同孵育胰腺腺泡细胞时,缓解了高浓度CCK-8诱导的对细胞淀粉酶分泌的抑制效应;SS降低了CCK-8诱导的胰腺腺泡细胞内胰蛋白酶的活性（P〈0.05）。结论SS稳定了骨架蛋白的结构,阻止了腺泡细胞内胰蛋白酶原的激活,这可能是SS对CCK-8诱导的急性胰腺炎腺泡细胞损伤的保护机制之一。Objective To investigated the effect of somatostatin（SS）on cholecystokinin-induced alterations of exocrine function and the redistribution of the F-actin in the pancreatic acinar cells.MethodsIn vitro,isolated rat pancreatic acini were divided into 12 groups：normal control group,different concentrations ofcholecystokinin-octapeptide groups（CCK-8）of 10^-12,10^-11,10^-10,10^-9 or 10^-8 mol/L,SS group（10^-7mol/L）,SS and different dose CCK-8 groups.The acinar cells were stimulated with different concentrations of CCK-8 for 30 min in the presence or absence of 10^-7 mol/L SS.Amylase and trypsin activity were measured using corresponding assay kits,and F-actin distribution in pancreatic acinar cells were detected by laser confocal microscopy.ResultsSS stabilized the structure of cytoskeleton in acinar cells,and remarkably increased the ratio of subapical/basolateral F-actin（P〈0.05）;Exposure to low concentrations of CCK-8（10^-11 to 10^-10 mol/L）in the presence of SS,the CCK-induced amylase release were significantly inhibited（P〈0.05）.However,at higher concentrations of CCK-8（10^-9 to 10^-8 mol/L）,the CCK-induced inhibition of amylase release was not observed as SS present,and SS significantly inhibited CCK-stimulated trypsin activity in acinar cells（P〈0.05）.ConclusionSS stabilizes the cytoskeleton morphology in the acinar cells,and prevents the trypsinogen activation,which might be one of the protective mechanisms of SS on CCK-induced acute injury in acinar cells.

关 键 词：生长抑素 胰腺 胆囊收缩素 肌动蛋白类 

分 类 号：R322.491[医药卫生—人体解剖和组织胚胎学] R329.2[医药卫生—基础医学]