饲料中添加水解鱼蛋白对半滑舌鳎稚鱼生长及生理生化指标的影响  

EFFECT OF FISH PROTEIN HYDROLYSATE LEVELS ON GROWTH PERFORMANCE AND BIOLOGICAL AND PHYSIOLOGICAL PARAMETERS IN TONGUE SOLE (CYNOGLOSSUS SEMILAEVIS GüNTHER, 1873) POST-LARVAE

作　　者：柳旭东[1,3] 梁萌青[1] 张利民[2] 王际英[2] 常青[1] 王家林[1] Yannis Kotzamanis 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]山东省海洋水产研究所,烟台264006 [3]山东升索渔用饲料研究中心,烟台265500 [4]希腊国家海洋研究中心海水养殖研究所,雅典

出　　处：《水生生物学报》2010年第2期242-249,共8页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金项目(30771660);公益性行业(农业)科研专项(nyhyzx07-046-鲆鲽);国际科技合作项目(2008DFA31720);青岛市科技计划项目(08-1-3-26-jch)(07-2-3-24-hz);中国水产科学研究院黄海水产研究所基本科研业务费专项资金项目资助

摘　　要：试验研究了饲料中添加不同水平(0%、20%、40%和60%)的水解鱼蛋白(Fish potein hdrolysate,FPH)对半滑舌鳎(Cynoglossus semilaevis Günther)稚鱼生长及生理生化指标的影响。28d饲养实验结束后,对试验鱼生长指标、鱼体消化酶活性、碱性磷酸酶(AKP)及肠道发育情况进行分析。结果显示:实验鱼的存活率为64.44%—78.88%,其中20%与40%添加组存活率显著高于其他两组(P<0.05);实验鱼的特定生长率(SGR)随饲料中FPH添加水平升高呈下降趋势,20%添加组特定生长率最高,显著高于其他3组(P<0.05),且60%添加组与对照组(FM)之间无显著差异(P>0.05);各试验组鱼体消化酶(脂肪酶、淀粉酶、胃蛋白酶和胰蛋白酶)的比活力在14d与28d时的强弱关系不一致。鱼体碱性磷酸酶比活力大小依次为:20%组>40%组>对照组>60%组,各组间差异均显著(P<0.05);半滑舌鳎肠道微绒毛长度和黏膜厚度均以20%添加组为最优,显著优于其他3组(P<0.05)。以上结果表明,在实验条件下,饲料中FPH的添加水平为20%时,能提高半滑舌鳎稚鱼的存活率及生长性能,并增强其对营养物质的吸收能力。This study evaluated the effects of fish protein hydrolysate inclusion in the diet of tongue sole （Cynoglossus semilaevis Günther, 1873） post-larvae on growth performance, the activities of alkaline phosphatase （AKP） and the di-gestive enzymes： pepsin, trypsin, lipase and amylase and intestine development in the fish. Four experimental diets were formulated by supplementing 0% （FM）, 20% （FPH-20）, 40% （FPH-40） and 60% （FPH-60） of FPH in the diet. Each diet was randomly assigned to triplicate groups of fish, and each group was stocked with 60 tongue sole post-larvae （30 days post-hatching, with an initial dry weight of 6.47±0.38 mg） in 12 fiberglass tanks （50 cm×40 cm×20 cm）. Fish were fed four times daily （05：00, 10：00, 15：00 and 21：00） to visual satiation for 28 days. During the feeding trial, water tempera-ture and salinity were maintained at （22±1℃） and 30—32, respectively. At the end of the 28-day growth experiment, survivals of the fish were relatively high and ranged from 64.44% to 78.88%. Fish fed diets with 20% and 40% FPH showed significantly higher survival rates than other two groups （P0.05）. Compared to the control, 20% FPH supple-mentation in the diet improved growth of the tongue larvae while high dose of FPH depressed that concerning the values of specific growth rate （SGR）; the activities of digestive enzymes, such as lipase, amylase, pepsin and trypsin showed no obvious trends among all treatments between 14 and 28 d, respectively; the AKP activity in all groups were ordered as follows （P0.05）： 20% FPH40% FPH0% FPH60% FPH; fish fed the diet with 20% FPH showed significantly higher intestinal villus and thicker mucosa than the other three groups （P0.05）. In this study, the diet with 20% of the FPH gave the best results in terms of growth, survival and intestinal development in tongue sole post-larvae, indicating FPH had a nutritional value equivalent to fish meal （FM）.

关 键 词：水解鱼蛋白 半滑舌鳎稚鱼 消化酶 碱性磷酸酶 肠道组织 

分 类 号：S963[农业科学—水产养殖]