伪柔弱拟菱形藻复合群的形态分类学研究  

THE STUDY ON MORPHOLOGICAL TAXONOMY OF PSEDUO-NITZSCHIA PSEUDODELICATISSIMA COMPLEX

作　　者：李扬[1] 何利娜[1] 马艳艳[1] 吕颂辉[1,2] 

机构地区：[1]华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,广东省高等学校生态与环境科学重点实验室,广州510631 [2]暨南大学理工学院,赤潮与水环境研究中心,广州510632

出　　处：《水生生物学报》2010年第2期302-311,共10页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(30700046);广东省自然科学基金(7300190)资助

摘　　要：于2007年夏季(8月)在广东省大亚湾海域观察到伪柔弱拟菱形藻复合群的6个种类:花形拟菱形藻(P.caciantha Lundholm,Moestrup & Hasle)、靓纹拟菱形藻(P.calliantha Lundholm,Moestrup & Hasle)、尖细拟菱形藻[P.cuspidata(Hasle)Lundholm,Moestrup & Hasle]、曼氏拟菱形藻(P.mannii Amato & Montresor)、伪柔弱拟菱形藻[P.pseudodelicatissima(Hasle)Lundholm,Hasle & Moestrup]和中华拟菱形藻(P.sinica Qi&Wang),其中花形拟菱形藻、靓纹拟菱形藻和曼氏拟菱形藻为我国新记录种类。对该复合群种类的形态学特征、生活习性和生态分布进行了观察和描述,并对其种类之间的形态学特征进行了比较研究。Increasing evidence show that cryptic and pseudo-cryptic species are widespread in several diatom taxa. This is the case with the genus Pseudo-nitzschia, which includes chain-forming pennate diatoms that bloom both in coastal and open ocean waters. In the last years, up 10 new species have been described on the basis of morphological and mo-lecular investigations, and some of them are only distinguishable on the basis of minute ultrastructural features of their siliceous frustulae. This is the case of P. pseudodelicatissima complex. It has been confirmed that P. pseudodelicatissima complex was a kind of pelagic diatom species which distribute commonly in Chinese coastal waters. Because of few subtle morphological features which just could be observed under electronic microscope （EM） among P. pseudodelica-tissima complex taxa, it is greatly difficult to reach accurate identification and there are many debates at present. For clarifying the species diversity of P. pseudodelicatissima complex and its dominant species in Daya Bay, water and net samples were collected from six stations in summer （August）, 2007. All samples were fixed with Lugo’s solution in situ and brought back to the laboratory. Then, they were concentrated to a final volume about 2—10 mL by settlement over-night for several times. These samples could be observed and analyzed under light microscope. For observation under transmission electron microscope, subsamples were taken and treated through several steps. Firstly, after dropped into same volume of concentrated H2SO4, these samples were boiled in the water bath for about 15—20 minutes to remove the organic materials. Secondly, these samples should be washed to neutrality with distilled water for several times. Then, these samples could be observed, identified, photographed and counted under transmission electron microscope. Including six species, P. caciantha Lundholm, Moestrup Hasle, P. calliantha Lundholm, Moestrup Hasle, P. cus-pidata （Hasle） Lundholm, Moestrup Hasle, P. man

关 键 词：伪柔弱拟菱形藻复合群 形态学特征 新记录种类 大亚湾 

分 类 号：Q94[生物学—植物学]