Double knockouts in human embryonic stem cells  

作　　者：Jizhong Zou Rory Cochran Linzhao Cheng 

机构地区：[1]Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA [2]Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA [3]Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

出　　处：《Cell Research》2010年第3期250-252,共3页细胞研究（英文版）

摘　　要：Gene targeting is the method of precise genome modification that utilizes the innate DNA repair mechanism in cells, known as homologous recombination. At its genesis, gene targeting in mouse embryonic stem （ES） cells was a breakthrough for genetics and developmental biology. Its application has enhanced researchers＇ ability to study gene function and model human disease, which was recognized by the Nobel Prize in Physiology or Medicine 2007.

关 键 词：人类胚胎干细胞 小鼠胚胎干细胞 诺贝尔生理学或医学奖 基因打靶 发育生物学 基因改造 细胞修复 同源重组 

分 类 号：Q813[生物学—生物工程] Q954.4