干细胞因子诱导神经干细胞定向迁移  

Stem Cell Factor Induces Directional Migration of Neural Stem Cells

作　　者：周春雷[1] 魏友华[1] 刘靖[1] 郑彦文[1] 张俊克[1] 王丹[1] 吴丹[1] 苏军[1] 张焕相[1] 

机构地区：[1]苏州大学医学部细胞生物学系江苏省干细胞研究重点实验室,苏州215123

出　　处：《中国细胞生物学学报》2010年第1期56-60,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金项目(No.30671041);国家重点基础研究发展规划(973计划)(No.2005CB623906);江苏省高校自然科学重大基础研究项目(No.06KJA18025)资助~~

摘　　要：神经干细胞(neural stem cells,NSCs)能够定向追踪胶质瘤,然而其内在作用机制及影响因素尚不明了。本实验运用Boyden chamber及Dunn chamber来研究干细胞因子(stem cell factor,SCF)诱导C17.2神经干细胞的趋化性迁移。Boyden chamber结果显示,下室加入SCF后迁移至膜下方的C17.2细胞数显著多于对照组;Dunn chamber结果显示,仅在外槽加入SCF的C17.2细胞迁移速率及迁移效率显著高于内外槽均加入SCF的细胞,单个细胞迁移轨迹分析表明仅在外槽加入SCF后细胞朝着浓度梯度方向迁移而内外槽均加入SCF后细胞的迁移则无规律。这些结果表明,SCF能够诱导C17.2细胞的定向迁移,为进一步了解神经干细胞定向追踪胶质瘤提供了理论依据。Neural stem cells （NSCs） can directly migrate towards glioma. However, the mechanisms and factors that regulate migration are not well understood. In this study, we investigated stem cell factor （SCF） induced C17.2 cells migration by Boyden chamber and Dunn chamber. Compared with the control, the results of Boyden chamber showed that the number of the ceils migrated to the lower well through a filter of 8-mm pore size was significantly higher than control when the SCF was added into the lower well. The Dunn chamber results indicated both the migration speed and the forward migration index （FMI） of cells exposed to SCF were significantly greater than those of cells exposed to a uniform concentration of SCF. The cell migration pattern also displayed a directed migration behavior towards the source of SCF in concentration gradients of SCF, while cells migrated randomly under conditions of uniform SCF distribution. These results illustrate that SCF induces the directional migration of C17.2 cells via the increase in the migration efficiency and the migration speed of these cells, which would provide data to understand the mechanisms of glioma-induced migration of NSCs.

关 键 词：神经干细胞 干细胞因子 趋化性 迁移 

分 类 号：Q813[生物学—生物工程]