猪卵母细胞体外成熟过程中的减数分裂进程  

The Meiotic Progress of Porcine Oocytes Matured in Vitro

作　　者：黄雅琼[1,2] 石德顺[1] 张晓溪[1] 张顺[1] 陈旭健[2] 李家洲[2] 曾诗媛[2] 阮桂文[2] 赵仕花[2] 谭世俭[1] 

机构地区：[1]广西大学动物繁殖研究所,南宁530005 [2]玉林师范学院,玉林537000

出　　处：《中国细胞生物学学报》2010年第1期84-90,共7页Chinese Journal of Cell Biology

基　　金：国家高技术研究发展计划(863计划)项目(No.2007AA100505;No.2008AA101006);广西壮族自治区教育厅科研项目[桂教科研(2009)25号;No.200911MS203]资助~~

摘　　要：本研究主要探讨猪卵母细胞体外成熟过程中的减数分裂进程和卵母细胞的发育潜力。直径>2 mm的卵泡内的卵母细胞体外培养16 h,大部分发生生发泡破裂(germinal vesicle breakdown,GVBD),卵母细胞GV期的比率由0 h的68.00%下降到16 h的15.38%。成熟培养20 h时,终变期(diakinesis,DK)的比率达到峰值(27.06%),而MI的峰值(69.66%)出现在培养后28 h;成熟培养32～44h时,Ana-Ⅰ/Tel-Ⅰ期的比率达到最高(53.33%);成熟培养48 h时,MII期的比率达到最大值(63.64%)。直径<2 mm、2～6mm和>6 mm卵泡的卵母细胞体外成熟培养48 h的成熟率(排出第一极体,PB1)分别为5.12%,66.62%和46.71%;孤雌激活后的分裂率分别为2.86%,72.11%和58.69%;囊胚率分别为0、28.62%和23.46%。直径<2 mm和>6 mm卵泡的卵母细胞的卵裂率明显低于直径2～6mm卵泡的卵母细胞的卵裂率(P<0.05);卵泡直径2～6 mm组和卵泡直径>6 mm组的囊胚率没有显著差异(P>0.05)。研究结果将为猪卵子的核成熟进程和控制减数分裂的研究提供理论依据。The meiotic progress and developmental competence of porcine oocytes matured in vitro were investigated in the present study. Most of oocytes from follicles over 2 mm in diameter were taken place germinal vesicle breakdown （GVBD） at 16 h after in vitro maturation （IVM）, in which the proportion of oocytes in the GV stage was decreased from 68.00% to 15.38%. At 20 h after IVM, the percentage of oocytes in the diakinesis stage was increased to 27.06%, and most of oocytes （69.66%） matured to the MI stage at 28 h after IVM. At 32-44 h after IVM, the proportion of oocytes in the Ana-Ⅰ/Tel-Ⅰ stage was up to the peak value （53.33%）. The percentage of porcine oocytes in the MII stage was up to the peak value （63.64%） at 48 h after IVM. The maturation rates of oocytes （extruded the first polar body, PB1） from follicles in diameter of 〈2 mm, 2-6 mm and 〉6 mm were 5.12%, 66.62% and 46.71%, respectively. The cleavage rates of oocytes from follicles in diameter of 〈2 mm （2.86%） and 〉6 mm （58.69%） were significantly lower than that of oocytes from follicles in diameter of 2-6 mm （72.11%, P〈 0.05） after IVM and parthenogenetic activation. However, the blastocyst yield of oocytes from follicles in diameter of 2-6 mm （28.62%） was not different from that of oocytes from follicles in diameter over 6 mm （23.46%, P〉0.05）. These results will provide necessary data for investigating the meiotic process and regulation of meiosis in porcine oocytes.

关 键 词：猪 卵母细胞 减数分裂 体外成熟 

分 类 号：Q813[生物学—生物工程]