痕量血红素的化学发光分析  

Study on Chemiluminescence Determination of Trace Amount of Haem

作　　者：袁丁[1] 何治柯[2] 罗庆尧[2] 余席茂[2] 曾云鄂 

机构地区：[1]湖北三峡学院医学院 [2]武汉大学

出　　处：《中南民族学院学报（自然科学版）》1998年第2期25-26,共2页Journal of South-Central University for Nationalities(Natural Sciences)

摘　　要：提出牛血清蛋白（ＢＡＳ）敏化氯化血红素ＨＭ－ＫＯＨ－Ｈ２Ｏ２化学发光新体系，测定了痕量血红素，线性范围为８．０×１０－６～８．０×１０－９ｇ·ｍＬ－１．本法用于牛血中痕量牛血红素的直接测定，获得满意结果．A new chemiluminescence system haemin (HM) KOH H 2O 2 sensibilized by bovine serum albumin(BSA) is presented. Based on this reaction, the trace amount of HM in borine blood can be determind with good resules. The linear range is 8.0×10 -6 ~8.0×10 -9 g·mL -1 . The mechanism of chemiluminescence reaction is discussed preliminarily.

关 键 词：血红素 牛血清白蛋白 化学发光分析 

分 类 号：Q592.1[生物学—生物化学] R446.112[医药卫生—诊断学]