液相色谱-电喷雾离子阱质谱法研究人尿中1,5-二咖啡酰奎宁酸的代谢产物  

Study on the metabolites of 1,5-dicaffeoylquinic acid in human urine by LC-ESI/MS/MS assay

作　　者：刘江林[1,2] 袁丹[2] 丁建刚[1,3] 董晓娜[1,2] 窦桂芳[1] 吴卓娜[1] 孟志云[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850 [2]沈阳药科大学中药学院,沈阳110016 [3]泰山医学院药学院,山东泰安271106

出　　处：《中国临床药理学杂志》2010年第3期217-220,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家科技部国际科技合作重点项目计划基金资助项目(2005DFA30080)

摘　　要：目的研究健康受试者口服1,5-二咖啡酰奎宁酸后尿液中的代谢产物。方法健康受试者每人口服1,5-二咖啡酰奎宁酸600mg,收集0-24h的尿样,经C18小柱固相萃取纯化后,用液相色谱-电喷雾离子阱质谱联用技术对人尿中的代谢产物进行分析鉴定。结果在人尿中发现了1,5-二咖啡酰奎宁酸的甲基化、葡萄糖醛酸化及甲基-葡萄糖醛酸化代谢产物共28个。其中,有2个代谢产物结构经标准品对照得到确证。结论甲基化、葡萄糖醛酸化和异构化反应是1,5-二咖啡酰奎宁酸在人体内的3种重要代谢途径。Objective To investigate metaholites of 1,5-dicaffeoylquin- ic acid （ 1, 5-DCQA） in human urine after oral administration of 1, 5- DCQA. Methods Urine samples were collected 0 -24 h after the oral administration of 600 mg dose of 1, 5-DCQA, then the samples were purified through Cls solid-phase extraction cartridge. The purified samples were analyzed by liquid chromatography and tandem electrospray ionization ion trap mass spectrometry （LC-ESI/MS/MS）. Results A total of 28 metabolites were discovered, including methylated, glucuronidated and methyl-glucuronidated metabolites. Two important metabolites were determined by comparing with synthesized standards. Conclusion These results suggest that methylation, glucuronidation and isomerization are three important metabolic pathways of 1, 5-DCQA in human.

关 键 词：液相色谱-电喷雾离子阱质谱 1 5-二咖啡酰奎宁酸 代谢产物 

分 类 号：R969.1[医药卫生—药理学] R978.7[医药卫生—药学]