清咽滴丸抗常见呼吸道病毒的实验研究  

Antiviral Activity of Qingyandiwan on Common Respiratory Trace Virus

作　　者：刘剑[1,2] 刘丽华[3] 孟庆军[3] 李晓眠[1] 苏文琴[4] 李晶哲[1] 

机构地区：[1]天津医科大学微生物学教研室,天津300070 [2]协和干细胞基因工程有限公司,天津300384 [3]天津中新药业集团股份有限公司第六中药厂,天津300401 [4]海南医学院,海口571101

出　　处：《中国药学杂志》2010年第7期519-523,共5页Chinese Pharmaceutical Journal

基　　金：天津市现代中药大品种群系统开发项目(06FZZDSH00406);天津市科委科技项目专项资金

摘　　要：目的研究清咽滴丸对呼吸道常见病毒的抑制作用。方法应用MTT方法,检测清咽滴丸对单纯疱疹病毒(HSV-1)和腺病毒(ADV11)的直接抑制作用、侵入细胞的抑制作用。应用鸡胚培养法,检测清咽滴丸体外灭活流感病毒的作用。建立ICR小鼠感染流感病毒模型,观察并记录小鼠的死亡情况,检测肺指数、肺脏病理改变及T、B淋巴细胞增殖情况和脾指数。结果①清咽滴丸对HSV-1和ADV11有直接抑制作用并且可抑制其侵入细胞。②鸡胚实验结果显示,清咽滴丸具有明显体外灭活病毒的作用。③动物实验结果显示,清咽滴丸不能降低动物的死亡率,但却可明显延长其平均存活时间;对肺脏有较好的抗炎作用;有刺激免疫细胞增殖,调节机体免疫功能的作用。结论清咽滴丸有明确的体外灭活病毒和一定的抗流感病毒鼠肺适应株(FM1)和调节机体免疫力的作用。在抗HSV-1和ADV11实验中,表现出确切的直接抑制作用和阻断病毒侵入细胞的作用。OBJECTIVE To investigate antiviral activity of Qingyandiwan on common respiratory trace virus including HSV-1, ADV11 and influenza virus FM1 （Flu）. METHODS MTT Colorimetric assay was used to evaluate the direct repression, invade inhibition of Qingyandiwan. The antiviral effects of extract of Qingyandiwan on influenza virus FM1 were observed in chick-embryo culture and ICR mice. The death rate, lung index, pulmonary pathological changes and the spleen index were observed. The spleen T and B lymphocyte proliferation experiment were carried out. RESULTS ①Qingyandiwan inactivated HSV-1 and ADV11 directly and inhibited the virus invading into cellur. The result of direct repression effect were as follows： IC50 was 201.66 μg·mL^-1 and TI was 14. 05 for anti-HSV-1, and the IC50 was 523.76 μg·mL^-1 and TI was 4. 68 for anti-ADV11. The results of cellular inhibition effect were as rollows： the IC50 was 213. 39/μg·mL^-1 and TI was 11.49 for anti-HSV-1, and the IC50 was 400. 88 μg·mL^-1 and TI was 6. 69 for anti-ADV11. ②Qingyandiwan showed significant inactivation of influenza virus FM1 in chick-embryo culture. ③The mortality rate of the model wasn＇t reduced（ P 〉 0.05 ）. However, the live time was prolonged significantly （ P 〈 0.01 ）. The anti-inflammatory effect on pneumonia was observed, and the immunological cells were irritated which proved Qingyandiwan had strengthening function on immune system. CONCLUSION Qingyandiwan showed significantly inactivation activity on influenza virus FM1 in vitro and strengthening function on immune system of mouse models. The empirical study of antiviral activity on HSV-1 and ADVI 1 showed that Qingyandiwan could inhibit and inactivate HSV-1 and ADV11 directly.

关 键 词：清咽滴丸 单纯疱疹病毒1型 腺病毒11型 流感病毒鼠肺适应株 抗病毒作用 

分 类 号：R965.1[医药卫生—药理学]