用生物测定法研究肉苁蓉多糖在体外的吸收转运  

Absorption and Transportation of Cistanche deserticola Polysaccharides Using Bioactivity Assay in Vitro

作　　者：齐云[1] 蔡润兰[1] 王翔岩[1] 李蒙[1] 李晓红[1] 杨美华[1] 

机构地区：[1]中国医学科学院一北京协和医院药用植物研究所,北京100193

出　　处：《中国药学杂志》2010年第7期527-530,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30672659);宁夏回族自治区科技攻关计划项目(05GG-10807)

摘　　要：目的用生物测定法考察肉苁蓉多糖(CDPS)在Caco-2细胞模型的吸收转运。方法用培养于Transwell上的Caco-2细胞模型研究CDPS的吸收转运;采用生物活性测定法测定CDPS在接受侧介质中的含量。结果Caco-2细胞单层极性与完整性符合实验要求,CDPS在1.87～40mg·mL-1内,与RAW264.7细胞NO分泌呈明显量效关系,转运量随供侧(AP侧)浓度的增加而增大,不同浓度CDPSPapp(A-B)均小于1×10-7cm.s-1。结论采用生物活性测定CDPS的Papp具可行性,该药属吸收不良药物,口服吸收率应小于1%。OBJECTIVE To study the absorption and transportation of Cistanche deserticola polysaccharides （CDPS） in Caco-2 monolayer model by utilizing bioactivity assay. METHODS The transwell filter inserts on culturing Caco-2 cell monolayer was used to observe the absorption and transportation of CDPS. Bioactive assay approach was used to determine the content of CDPS in the receiver compartment. RESULTS The integrity and polarity of Caco-2 cell monolayer were conformed to the experiment requirements. CDPS in the concentration range of 1.87 - 40 mg · L^-1 enhanced the release of NO of RAW264. 7 cell in a dose-dependent manner. With the loading concentration rising, the transportation of quantity of CDPS was increased. The Papp Of CDPS for AP-BL was less than 1 × 10^-7 cm·s^-1. CONCLUSION It is feasible to determine Papp, of CDPS by bioactivity assay. CDPS is a poorly absorbed drug and its oral absorbability should be less than 1%.

关 键 词：肉苁蓉多糖 CACO-2细胞单层模型 表观渗透系数 吸收转运 

分 类 号：R945[医药卫生—微生物与生化药学]