山东地区结核分枝杆菌临床菌株基因型特征研究  

Study on molecular characteristics regarding DNA genotype of Mycobacterium tuberculosis clinical strains in Shandong

作　　者：邓云峰[1] 张延安[1] 郑建礼[1] 景辉[1] 王燕[1] 王海英[1] 马欣[1] 刘志敏[1] 

机构地区：[1]山东省胸科医院,济南250013

出　　处：《中华流行病学杂志》2010年第3期321-323,共3页Chinese Journal of Epidemiology

基　　金：山东省医药卫生科技发展计划（2005JW036,2009HW077）

摘　　要：目的探讨山东地区结核分枝杆菌临床分离菌株的基因型特征，评估不同基因型分布对于耐药结核菌近期传播的影响。方法在山东地区选取13个结核病防治机构作为监测哨点收集临床分离菌株和相关信息，应用分枝杆菌散在重复单位（MIRu）技术分析结核分枝杆菌DNA多态性。结果1年的研究期内共获得558株结核分枝杆菌，对12个MIRU位点进行检测共产生143个基因型，其中成簇基因型66个，成簇率86．2％。74．6％的菌株属于北京家族，177个（31．7％）菌株属于山东基因型。耐多药菌株的近期感染估计值明显低于敏感菌株。结论山东地区结核分枝杆菌具有明显的基因多态性，山东基因型菌株在人群中具有较强的传播能力。Objective To establish the molecular characteristics of Mycobacterium tuberculosis and on factors influencing the recent transmission of drug resistant isolates in Shandong. Methods Mycobacterium tuberculosis isolated from active pulmonary tuberculosis patients of 13 counties were genotyped by mycobacterial interspersed repetitive units （MIRU） methods. Results 12 loci of MIRU were detected in 558 isolates and a total of 143 MIRU patterns were confirmed. 66 isolates had distinct patterns, and 481 （86.2%） strains were in clusters. Shandong cluster included 177 strains with 74.6% of the isolates belonged to Beijing family. The recent transmission index of multi-drug resistance strains was in lower level, comparing to the susceptible strains. Conclusion Our results showed that the Shandong cluster isolates had capacities of facilitating person-to-person transmission and high level of drug resistance.

关 键 词：结核 分枝杆菌 流行病学 基因分型 耐药性 

分 类 号：R450[医药卫生—治疗学]