IFN-γ＋874基因多态性与慢性HBV和／或HCV感染及不同临床转归的关系  

Association between IFN--γ＋874 polymorphisms and the clinical outcomes of hepatitis B and/or hepatitis C virus infection

作　　者：高秋菊[1] 刘殿武[2] 张世勇[3] 贾敏[4] 吴丽红[1] 

机构地区：[1]白求恩军医学院预防医学教研室,石家庄050081 [2]河北医科大学公共卫生学院 [3]石家庄市疾病预防控制中心 [4]河北省人民医院

出　　处：《中华流行病学杂志》2010年第3期324-328,共5页Chinese Journal of Epidemiology

摘　　要：目的研究HBV和／或HCV感染及其不同临床转归者干扰素-γ（IFN-γ）＋874基因多态性（SNP），探讨其免疫遗传机制。方法对河北省赵县某农村HBV和／或HCV感染者及对照共277人采集空腹静脉血，用ELISA检测抗-HBV、抗-HCV生物标志物，筛选出HBV重叠HCV感染79例、单纯HBV感染69例、HCV感染55例和对照74例。用RT-nPCR检测HCVRNA，BeckmanLX-20全自动生化仪检测肝功能丙氨酸氨基转移酶（ALT），用PCR—SSP检测IFN．7＋874T／ASNP，分析IFN-γ＋874T／ASNP与HBV和／或HCV感染、不同临床转归、ALT和HCVRNA表达的关系。结果（1）不同感染类型即单纯HBV、HCV感染和重叠感染者＋874AA频率明显高于对照，＋874TA频率明显低于对照（X：16．15，P=0．01），OR值及95％C1分别是3．22（1．43～7．25）、2．70（1．24～5．92）、4．02（1．88～8．55），各感染类型间差异无统计学意义（t=1．97，P=0．73）；＋874T／A等位基因频率在各组间差异无统计学意义（X2=4．87，P=0．18）；（2）不同临床转归即轻型、中重型肝炎和肝硬化组＋874AA频率明显高于对照组、＋874TA频率明显低于对照组（χ2=14．17，P=0．03），OR值（95％CI）分别是3．09（1．51～6．33）、3．85（1．70—8．70）、3．14（1．08～9．17）。＋874T／A等位基因频率与不同临床转归无统计关联（f=6．07，P=0．11）；（3）IFN-γ＋874SNP与HCV病毒复制无统计学关联（χ2=2．36，P=0_31），与ALT水平无统计学关联（χ2=0．15，P=0．93）。结论IFN-γ＋874T／ASNP与HBV和／或HCV感染者慢性化及不同临床转归有一定关联，IFN-γ＋874AA能增加HBV和威HCV感染及其临床转归的危险，＋874TA则减低其感染和临床转归风险。Objective To explore the association between polymorphisms of interferon- gamma gene intron 1 at position ＋ 874 （IFN-γ, ＋ 874） gene and the susceptibility of HBV and/or HCV infection with different clinical outcomes. Methods IFN-γ ＋ 874 gene SNP were detected in 277 subjects including 79 chronic HBV/HCV coinfections, 69 individuals only with HBV infection, 55 individuals only with HCV infection and 74 controls, by sequence specific primers-PeR （SSP-PCR）. Hepatocellular injury as suggested by alanine aminotransferase （ALT） was detected by Beckman LX-20. The status of viral particles in serum was determined by RT-nPCR. The possible association of the polymorphism of IFN-γ 4874 with the susceptibility of HBV and/or HCV infection and the outcome of these infections were analyzed. Results （1） IFN-γ ＋ 874 AA frequency in individuals with chronic HBV, HCV, HBV/HCV coinfections were significant higher than that in controls （χ2= 16.15, P=0.01 ） ; OR （95% el） of IFN-γ ＋ 874 AA in chronic infection with HBV,HCV, HBV/HCV coinfections appeared to be 2.70 （1.24-5.92） , 3.22 （1.43-7.25） and 4.02 （1.88- 8.55 ） compared with -874 TA. No significant differences were found among HBV, HCV, HBV/HCV coinfections （χ2= 1.97, P=0.73）. There were no significant association of IFN-γ Jr 874 A/T allele frequency with HBV and/or with HCV infection （χ2=4.87, P=0.18）. （2）The clinical outcomes of mild chronic hepatitis （CH）, moderate/severe CH and cirrhosis with HBV and/or HCV infection were associated with IFN-γ ＋874 AA [p= 14.17 ,P=0.03 ; OR=3.09（ 1.51-6.33 ）, 3.85 （ 1.70-8.70）, 3.14 （1.08-9.17） ]. No significant relationships were found between IFN-γ ＋ 874 A/T allele frequency and the clinical outcome of HBV/HCV infection （χ2=6.07, P=0.11）. （3）There were no significant associations of IFN-γ ＋ 874 genotype/allele frequency with HCV duplication （χ2=2.36, P=0.31）. （4） There were no significant associations of IFN-γ ＋ 874 genotype/alle

关 键 词：病毒性肝炎 干扰素-Γ 单核苷酸多态性 

分 类 号：R512.62[医药卫生—内科学]