康莱特注射液对HL-60/ADR细胞周期调控蛋白P16、P63表达的影响  

The Effect of Kanglaite Injection on Expression of Cyclin Modulin P16,P63 in HL-60/ADR Cells

作　　者：余永莉[1] 李季蓉[1] 

机构地区：[1]遵义医学院珠海校区解剖组胚教研室,广东珠海519041

出　　处：《中国现代应用药学》2010年第3期202-205,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：贵州省卫生厅课题资助(2002-D181)

摘　　要：目的探索康莱特注射液对HL-60/ADR细胞周期调控蛋白P16、P63表达的影响。方法使用康莱特注射液作用于HL-60/ADR细胞,用细胞计数法检测康莱特注射液对HL-60细胞生长曲线的影响,MTT法检测阿霉素对康莱特注射液作用后细胞的抑制效应。康莱特注射液作用HL-60/ADR细胞一定时间后,再用一定浓度的阿霉素作用该细胞一定时间,流式细胞仪检测该细胞内的阿霉素浓度并分析细胞周期调控蛋白P16、P63的表达。结果各组细胞计数第6天达峰值,康莱特注射液各组细胞计数,较未加药组细胞计数低(P<0.05)。1mg·mL-1康莱特注射液作用HL-60/ADR细胞96h,阿霉素对该细胞的抑制效应较对康莱特注射液未作用的HL-60/ADR细胞抑制效应强(P<0.05);不同浓度的康莱特注射液作用HL-60/ADR细胞96h后,再用1.5μg·mL-1的阿霉素作用6h,该细胞内的阿霉素荧光强度平均值(127.2±4.8)较对照组(77.8±7.2)高(P<0.05),且具有剂量依赖关系;1mg·mL-1康莱特注射液作用HL-60/ADR细胞48h,HL-60/ADR细胞的细胞周期调控P16的平均值(898.46±30.50)较对照组(698.45±91.29)高(P<0.05);P63的平均值(837.53±42.75)较对照组(591.33±92.99)高(P<0.05)。结论康莱特注射液对HL-60细胞生长有抑制作用,可能有促进细胞对阿霉素的敏感性的作用。康莱特注射液还有上调HL-60/ADR细胞周期调控蛋白P16、P63的表达,此机制可能参与康莱特注射液抗肿瘤作用。OBJECTIVE To investigate the effect of Chinese medicine Kanglaite injection on expression of cyclin modulin P16,P63 in HL-60/ADR cells.METHODS HL-60/ADR cells during logarithmic growth phase were treated with Kanglaite injection,growth curve was based on cell counting;the inhibitory effect of adriamycin（ADR） on Kanglaite injection-treated HL-60/ADR cells was tested by MTT assay;the concentration of intracellular adriamycin and the expression of P16,P63 were detected by flow cytometry.RESULTS In each group,live cell count reached a peak on the 6th day,compared with control group,the Kanglaite injection-treated cell count was lower with statistical significance（P〈0.05）.Treated HL-60/ADR cells with 1 mg·mL-1 Kamglaite for 96 h,the inhibitory effect of adriamycin on the cells were greater than that of control groups（P〈0.05）.After treatment with different concentration of Kanglaite injection,the intrancellular ADR fiourescence of 1.5 μg·mL-1 ADR-treated for 6 h were statistical higher than that of control group（P〈0.05）.Treated HL-60/ADR cells with 1 mg·mL-1 Kamglaite for 48 h,compared with the control group,the expression of P16,P63 were all significantly increased（P〈0.05）.CONCLUSION HL-60/ADR cells exposed to Kanglaite injection resulted in dose-depentent inhibitory,Kanglaite injection could increase the sensitivity of HL-60/ADR cells to ADR.Kanglaite injection could increase the expression level of P16,P63 in HL-60/ADR cells,that maybe involved in the anti-tumor mechanics of Kanglaite injection.

关 键 词：康莱特注射液 HL-60/ADR细胞 细胞周期 P16 P63 

分 类 号：R965.2[医药卫生—药理学]