HPLC测定注射用亚锡依替菲宁的含量  

Determination of Etifenin and Stannous Chloride for Injection by HPLC

作　　者：陈志明[1] 虞燕华[1] 吴二明[1] 李卫一[1] 汪洋[1] 

机构地区：[1]江苏省原子医学研究所,江苏无锡214063

出　　处：《中国现代应用药学》2010年第3期258-260,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立用HPLC测定注射用亚锡依替菲宁(EHIDA药盒)含量的方法。方法采用Waters C18(4.6mm×150mm,5μm)的色谱柱,以甲醇-水-磷酸(55∶45∶0.075)为流动相,检测波长为220nm,流速1.0mL·min-1。结果依替菲宁在32～384μg·mL-1内成线性关系,Y=1789041X+91432(r=0.99992)平均回收率为98.77%,RSD为0.43%。结论本方法操作简便,结果准确,可用于EHIDA药盒含量的快速定量分析。OBJECTIVE To establish a method for determination of etifenin and stannous chloride for injection（EHIDA kit）.METHODS HPLC was adopted with Waters C18（4.6 mm×150 mm,5 μm） column,methanol-water-phosphoric acid（55∶45∶0.075） was used as the mobile phase.The flow rate and detection wavelength was 1.0 mL·min^-1,220 nm,respectively.RESULTS The linear rage of etifenin were 32-384 μg·mL-1,r=0.999 92,the average recovery was 98.77%,RSD was 0.43%（n=7）.CONCLUSION The method is simple,accurate and suitable for the determination of EHIDA kit.

关 键 词：高效液相色谱法 依替菲宁 含量测定 

分 类 号：R917.101[医药卫生—药物分析学]