清紫颗粒对小鼠被动皮肤过敏反应的影响  

Effect of Qingzi Keli on passive cutaneous anaphylaxis in mice

作　　者：凌科[1] 张建民[1] 关晶[1] 

机构地区：[1]首都儿科研究所附属儿童医院,北京100020

出　　处：《北京医学》2010年第4期316-318,共3页Beijing Medical Journal

摘　　要：目的探讨青紫颗粒的抗过敏反应。方法选择60只15-17g及10只30g昆明种小鼠,雌雄各半。取10只体重30g小鼠,两后脚掌各注入天花粉氢氧化铝凝胶混悬液0.05ml,7d后处死,取血清备用。取50只15-17g小鼠（实验组）,将上述血清稀释后在每只小鼠腹壁皮内注射0.06ml,余10只作为空白对照。将50只实验组小鼠随机分为5组：模型对照组,清紫颗粒高剂量组（0.194g/ml）、中剂量组（0.097g/ml）、低剂量组（0.0485g/ml）和马来酸氯苯那敏组（0.1mg/ml）。空白对照组、模型对照组给予生理盐水灌胃,其余各组均给予相应药物灌胃,连续给药7d后注射天花粉注射液24mg/kg,20min后处死,采用蓝斑面积法和比色法评价各组药物对小鼠被动皮肤过敏反应的抑制作用。结果清紫颗粒高、中、低剂量组均对小鼠被动皮肤过敏反应有抑制作用,清紫颗粒高剂量组和马来酸氯苯那敏组的抑制作用优于清紫颗粒的中、低剂量组。结论清紫颗粒具有良好的抗过敏作用。Objective To study passive cutaneous anaphylaxis（PCA） of Qingzi Keli. Methods Sixty 15-17g and ten 30g KM mice were used. Each 30g mice was injected with trichosanthin and aluminium hydroxide to get the standby serum. Sixty 15-17g mice were divided into test groups with fifty mice and blank drug group with ten mice. The test group mice were randomly distributed into five groups： model control group, high dose of Qingzi granules group, middle dose of Qingzi granules group, low dose of Qingzi granules group and chlorphenamine maleate group. After continuous intragastric administration of physiological saline group and corresponding drug for 7d,mice were injected trichosanthin 24mg/kg and killed respectively. Inhibitory effect of PCA in six groups were measured by blue-spot＇area and colorimetric analysis. Results High, middle and low dose Qingzi granules could inhibit PCA in mice, the effect of high-dose Qingzi granules and chlorphenamine maleate were stronger than that of middle and low-dose Qingzi granules. Conclusions Qingzi Keli showed a significant inhibition on passive cutaneous anaphylaxis （ PCA ）. It indicates Qingzi Keli has obvious antianaphylaxis effect.

关 键 词：清紫颗粒 抗过敏 被动皮肤过敏反应 

分 类 号：R285.5[医药卫生—中药学]