鸡胚法筛选具有抗鸡球虫病作用的Bt菌株  

Screening BT with Anti-coccidiosis Effect by Chick Embryos Method

作　　者：吴昌标[1,2] 吴莉莉[1] 林伯全[2] 

机构地区：[1]福建农林大学教育部生物农药与化学生物学重点实验室,福州350002 [2]福建农业职业技术学院,福州350119

出　　处：《中国农学通报》2010年第7期21-25,共5页Chinese Agricultural Science Bulletin

基　　金：福建省科技厅重点项目"苏云金芽孢杆菌晶体蛋白防治动物疾病的研究与应用"(2008N0050);福建省自然基金项目"苏云金芽孢杆菌防治鸡球虫病的机理研究"(2009j0110)

摘　　要：用鸡胚感染柔嫩艾美耳球虫子孢子作为苏云金杆菌晶体蛋白抗球虫活性测定的模型。每一鸡胚接种1×104个子孢子,于感染前24h注入苏云金杆菌晶体蛋白,以不同苏云金杆菌晶体蛋白对鸡胚卵囊所产生的抑制率的差异,判定苏云金杆菌的活性。结果表明,各种苏云金标杆菌晶体蛋白对球虫卵囊都有一定抑制作用,其中苏云金杆菌Bt6菌株作用尤其明显。表明此方法具有操作简单,结果可信及成本低廉的特点,可作为抗球虫苏云金杆菌的筛选方法。Chick embryos inoculated with sporozoites ofE. Tenella were used as a model for testing the activity of BT crystal proteins. Each embryo were inoculated by a dose of 1×104 pure sporozoites was injected with testing bt crystal proteins before 24 hours. The varying oocyst inhibition rates of embryos brought about by BT crystal proteins that were given at various doses were used as a criterion for determining the activity of bt crystal proteins. The results demonstrated that BT6 was the most effective isolate among the 15 strains. According to the experimental results mentioned above, we believe that the use of chicken embryo infection with E. Tenella sporozites for the determination of the activity of BT crystal proteins is an efficacious method, which offers some advantages, such as simplicity of operation, good accuracy and only small requirement of expenses.

关 键 词：鸡胚 抗鸡球虫病 苏云金杆菌 

分 类 号：S858[农业科学—临床兽医学]