人参根际土壤微生物RAPD反应体系的建立  

Establishment of RAPD Reaction System for Panax ginseng Rhizosphere Soil Microbial Community

作　　者：应益昕[1] 丁万隆[1] 李勇[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国农学通报》2010年第7期36-39,共4页Chinese Agricultural Science Bulletin

基　　金：高等学校博士学科点专项科研基金资助课题"人参栽培过程中土壤微生物群落结构及功能演变研究"(200800231060)

摘　　要：为建立适用于人参根际土壤微生物遗传多样性分析的RAPD反应体系;参考前人研究报道,优化土壤微生物RAPD反应体系及扩增程序,以满足人参根际土壤微生物遗传多样性分析的要求。利用建立的通用性良好的优化体系,对200条RAPD引物进行了筛选,共获得多态性高、扩增结果稳定的RAPD引物38条;用筛选出的RAPD引物分析了人参根际土壤微生物的遗传多样性,扩增条带清晰可辨。建立的RAPD反应体系通用性好,适合开展人参根际土壤微生物遗传多样性研究。To establish RAPD reaction system that fitted for genetic diversity study of Panax ginseng rhizosphere soil microbial community, and screen polymorphic RAPD primers by optimized RAPD reaction system;. According to reports published, optimize soil microbial RAPD reaction system and amplification program, and fitted for genetic diversity study ofP. ginseng rhizosphere soil microbial community. By optimized universal RAPD reaction system, 38 out of 200 RAPD primers having high polymorphism and amplified stably were identified. Furthermore, clear bands can be amplified by RAPD primers identified with genomic DNA extracted fromP. ginseng rhizosphere soils as PCR template. The established universal RAPD reaction system was fitted for genetic diversity study ofP. ginseng rhizosphere soil microbial community.

关 键 词：人参 根际土壤 微生物 RAPD 反应体系 

分 类 号：Q938.1[生物学—微生物学]