非洲菊组织培养基筛选研究  

Research on the Screening for Gerbera Tissue Culture Medium

作　　者：高绍良 周寒松 张素芳 

机构地区：[1]宁德职业技术学院,福建福安355000

出　　处：《中国农学通报》2010年第7期210-213,共4页Chinese Agricultural Science Bulletin

基　　金：宁德市科技课题"非洲菊组培快繁及高效无土栽培技术"(2005Z05)

摘　　要：采用不同的培养基配方对非洲菊花托进行组织培养试验,综合分析不同的培养基配方在非洲菊组培过程中对花托培养、继代增殖和壮苗生根的影响。结果表明在试验配方中非洲菊花托培养的最适组织培养基为MS+6-BA10mg/L+NAA1.0mg/L+糖30g/L+琼脂5%;继代增殖培养的最适组织培养基为MS+6-BA1.0mg/L+NAA0.2mg/L+糖30g/L+琼脂7%;无根苗壮苗生根的最适组织培养基均为1/2MS+NAA0.5mg/L+糖20g/L+琼脂7%。Using different culture medium formula on the tissue culture experiment forGerbera receptacle, a comprehensive analysis of the various culture medium formulas in the process ofGerbera tissue culture which effects the cultivation of callus induction, subculture, and strong sprout and rooting. It turns out that most appropriate tissue culture medium ofGerbera callus induction in the test formulations is MS ＋ 6-BA10mg/L ＋ NAA1.0mg/L ＋ sugar 30g/L ＋ agar 5% ; the most appropriate tissue culture medium for the subculture cultivation is MS ＋ 6-BA1.0mg/L ＋ NAA0.2mg/L ＋ sugar 30g/L ＋ agar7% ; the most appropriate tissue culture medium of strong sprout and rooting is 1/2MS ＋ NAA0.5mg/L ＋ sugar20g/L＋ agar 7%.

关 键 词：非洲菊 组织培养 培养基 

分 类 号：S3[农业科学—农艺学]