微卫星标记AY493302与缙云麻鸭蛋壳颜色的相关性分析  

Analysis on Correlation between Microsatellite Marker AY493302 and Eggshell Color of Jinyun Duck

作　　者：袁青妍[1] 陶争荣[1] 李国勤[1] 沈军达[1] 陈奕春 陈文标 王德前[1] 虞永亮 田勇[1] 原爱平[1] 卢立志[1] 

机构地区：[1]浙江省农业科学院畜牧兽医研究所,杭州310021 [2]缙云县畜牧兽医局,浙江缙云321400 [3]浙江五莲农牧有限公司,浙江缙云321403

出　　处：《中国农学通报》2010年第8期12-16,共5页Chinese Agricultural Science Bulletin

基　　金：国家"十一五"科技支撑计划课题"鸭健康养殖技术与工艺模式示范"(2006BAD14B06);浙江省重大科技攻关项目"特色家禽(蛋鸭;肉鸡)优良遗传资源开发利用研究"(2005C12005-01);浙江省重大国际合作项目"浙江省主要鸭种质资源多态性研究与应用"(2006C14015)

摘　　要：采用50只青壳蛋缙云麻鸭及30只白壳蛋缙云麻鸭的血液DNA对微卫星标记AY493302进行多态性分析,探讨该微卫星标记与缙云麻鸭蛋壳颜色的相关性,为鸭青壳性状的分子标记辅助选择提供理论依据和技术支持。结果微卫星标记AY493302在青壳蛋与白壳蛋缙云麻鸭之间的基因型分布差异极其显著(P=2.247E-12),表明该微卫星标记与缙云麻鸭的蛋壳颜色高度相关。其中基因型JJ(365bp/365bp)不仅在2种鸭中的频率差异极其显著(P<0.0001),而且只在青壳的缙云麻鸭中出现,在白壳的缙云麻鸭中尚未出现,表明基因型JJ(365bp/365bp)可以作为缙云麻鸭纯合青壳基因标记辅助选择的基因型。Microsatellite locus AY493302 was used to analyze the polymorphisms of 50 blue-eggshelled and 30 white-eggshelled Jinyun ducks for the correlation relationship between this marker and eggshell color of Jinyun ducks. The results showed that the genotype distributions of this marker between two eggshell color ducks were significantly different （P=2.247E-12）. This denotes that microsatellite AY493302 was highly correlated with eggshell color of Jinyun duck. The frequency of genotype JJ （365bp/365bp） was also significantly different between two eggshell color ducks （P〈0.0001）. Furthermore, this genotype existed only in blue-eggshelled Jinyun ducks, while not in white-eggshelled ones. These suggested that genotype JJ （365bp/365bp） can be used to select the homozygous blue-eggshelled gene of Jinyun duck in the form of assistant marker.

关 键 词：缙云麻鸭 微卫星 蛋壳颜色 

分 类 号：S813.3[农业科学—畜牧学]