不同浓度乙二醇和甘油对犬精液冷冻效果的影响  

Influence of Different Concentrations of Ethylene Glycol and Glycerol on Quality of Canine Frozen Sperm

作　　者：高雅[1] 罗桂河 李卫东 李复煌[4] 孟丹娜 康蕊[1] 秦秀蕊[1] 倪和民[1] 

机构地区：[1]北京农学院动物科技系,北京102206 [2]北京市昌平区兽医局,北京102200 [3]北京市昌平区动物卫生防疫站,北京102200 [4]北京市畜牧兽医总站,北京100010

出　　处：《中国农学通报》2010年第8期25-28,共4页Chinese Agricultural Science Bulletin

基　　金：国家"十一五""863计划"重点项目"家畜卵泡高效诱导发育技术研究与应用"(项目编号:2008AA101003);国家"十一五科技支撑计划"项目"肉羊高效育种关键技术的研究与应用"(项目编号:2008BADB2B04-4)

摘　　要：研究在常规冷冻稀释液中分别添加不同浓度的乙二醇作为保护剂,并与甘油作为冷冻保护剂进行对比,以优化犬精液冷冻方案,提高解冻后精子质量。在犬的常规精液冷冻稀释液中分别添加不同浓度的乙二醇(2%,4%,6%,8%,10%),经细管冷冻、解冻后通过分别评价其精子的活率、顶体完整率、畸形率等来筛选其最佳添加浓度。结果表明:添加浓度为6%的乙二醇时,冷冻后精子活力为0.37,顶体完整率为43%,显著好于其他处理组(P<0.05);添加浓度为4%的乙二醇时,冷冻后精子畸形率为15%,略优于其他处理组。6%浓度的乙二醇可代替甘油作为冷冻保护剂,且对犬的冷冻效果有显著的促进作用。Objective To optimize the freezing programs for canine semen, the different concentrations of ethylene glycol as protective agent were added in the conventional frozen diluted solution respectively, and compared with the groups added by glycerol as cryoprotectant. Methods The different concentrations of ethylene glycol（2%, 4%, 6%, 8%, 10%） were added in conventional frozen diluted solution of canine semen respectively, the post-thaw semen motility, the percentage of sperms with intact acrosome and the percentage of abnormality were respectively analyzed to estimate the quality of frozen semen. Results The sperm motility in the group with 6% ethylene glycol was 0.37, and its percentage of sperms with intact acrosome was 43%, much higher than those of the other groups（P〈0.05）. The percentage of abnormality of 4% ethylene glycol was 15%. Conclusion 6% of ethylene glycol could replace the glycerol as a cryoprotectant, and improve the effect of freezing canine sperm.

关 键 词：犬 乙二醇 甘油 冷冻精液 

分 类 号：S814.8[农业科学—畜牧学]