缺氮条件下燕麦根轴细胞的程序性死亡  

Programmed Death of Cortical Cells in Oat Roots under Nitrogen Deficiency

作　　者：张子义[1] 伊霞[1] 胡博[1] 胡云才[2] 刘景辉[1] 樊明寿[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]慕尼黑工业大学植物营养研究所,德国慕尼黑d85350

出　　处：《中国农学通报》2010年第8期175-178,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"氮;磷营养胁迫条件下植物根组织的程序性死亡"(30660086);现代农业产业技术体系建设专项资金

摘　　要：采用溶液培养的方法,比较了缺氮和充足供氮条件下燕麦根组织DNA提取液的电泳图谱以及根系分泌物中有机酸、游离氨基酸、可溶性糖含量的差别。缺氮燕麦根基部组织梯状DNA的出现证实缺氮条件下燕麦根轴皮层细胞发生了程序性死亡,过去发现的缺氮或缺磷植物根轴内的空腔或通气组织即是细胞程序性死亡的结果。而且在缺氮胁迫下,根基部细胞首先发生程序性死亡。燕麦根系分泌物的分析结果表明,根系的游离氨基酸和可溶性糖数量与根细胞的PCD无关,但缺氮燕麦根系总有机酸的分泌量显著大于供氮处理的结果意味着缺氮燕麦根轴解体细胞的大分子物质有可能以有机酸的形式释放。Oat roor tissue DNA electrophoresis, and organic acids, amino acids, soluble sugar in root exudations were compared between different N levels by using solution culture. DNA ladder appeared in proximal ends of oat roots suugested that programmed cell death happened in oat root with no N supply. Therefore the arenchyma or cavum formed in root cortex under N or P deficiencies can be deduced as results of cortical cell PCD. Additionally, the result of this research also suggested the PCD initiates in proximal part of root under N dificiency.The results that amino acids and soluble sugar concentrations in oat root exdudation under no N condition were not significantly higher than those under abundant N supplies suggested that amino acids and soluble sugar concentrations in oat root exdudation has nothing to do with root PCD. However, the reasult that more organic acids were exudated by oat roots with no N than those with N implied that organic acids exudated by oat root may relate to the PCD happened in root cortex.

关 键 词：燕麦 缺氮 程序性死亡 

分 类 号：Q945[生物学—植物学]