酶动力学方法在黄曲条跳甲抗性监测中的应用  

Application of Enzymatic Kinetics Method in Resistance Monitoring of Striped Flea Beetle,phyllotreta striolata(Coleoptera:Chrysomelidae)

作　　者：郑丽祯[1,2] 傅建炜[1,2] 李建宇[1] 林泽燕[2] 占志雄[1] 

机构地区：[1]福建省农业科学院植物保护研究所,福州350013 [2]福建农林大学应用生态研究所,福州350002

出　　处：《中国农学通报》2010年第8期226-229,共4页Chinese Agricultural Science Bulletin

基　　金：福建省自然科学基金"毒死蜱在黄曲条跳甲灾变中的作用及其机理研究"(B0610025)

摘　　要：利用酶标仪动力学方法测定4个菜区黄曲条跳甲的总酯酶、乙酰胆碱酯酶活性分布频率,并与浸叶法测定的药剂敏感性进行比较分析,结果表明利用酶标动力学方法能够快速测定黄曲条跳甲总酯酶、乙酰胆碱酯酶活性在不同活性区段的频率分布,通过分析总酯酶活性大于3.2D(450nm)/(min·insect)、乙酰胆碱酯酶活性小于0.016D(405nm)/(min·insect)的个体数可以快速监测黄曲条跳甲田间种群的药剂敏感性水平高低。Frequency distributions of general esterase and acetylcholinesterase in striped flea beetle,phyllotreta striolata,from 4 regions were detected with enzymatic kinetics method in a microplate reader, and were compared with the insecticide susceptibility tested by leaf dipping methods. The results indicated that the former method was a rapid detection of insecticide susceptibility of striped flea beetle by analyzing individual number on the general esterase with an activity of more than 3.2⊿D（450nm）（min·insect）, while acetylcholinesterase less than 0.016⊿D（405nm）（min·insect）.

关 键 词：酶标仪动力学方法 黄曲条跳甲 抗性监测 

分 类 号：S438[农业科学—农业昆虫与害虫防治]