硒对蚕豆胚根抗氧化酶系统的影响  

The Effect of Selenium on the Antioxidaut Enzyme System of Radicle Root of Vicia Faba

作　　者：张苗苗[1] 韩善华[1] 王亚男[1] 杜凤移[1] 王阚[1] 王安喜[1] 

机构地区：[1]四川师范大学生命科学学院,四川成都610066

出　　处：《四川师范大学学报（自然科学版）》2010年第2期239-242,共4页Journal of Sichuan Normal University（Natural Science）

基　　金：四川师范大学科研启动基金(037054)

摘　　要：采用水培方法,研究不同浓度Se4+对蚕豆胚根抗氧化酶系统的影响.结果表明,随着Se4+浓度的升高,SOD活性一直升高,而CAT活性下降;当Se4+浓度低于0.01μmol/L时POD活性随着浓度升高而下降,Se4+浓度高于0.01μmol/L时POD活性开始升高;Se4+浓度低于0.005μmol/L时MDA含量随浓度升高而降低,Se4+浓度高于0.01μmol/L时MDA含量开始升高.对POD同功酶的聚丙烯酰胺凝胶电泳结果分析表明,不同浓度的Se4+处理蚕豆根POD同功酶没有特异性条带的表达,但活性强弱不同,有的带已经消失了.The authors investigated antioxidant enzyme system in the radicle of Vicia Faba treated with different concentrations of Se4＋.The results indicated that SOD activity increased and CAT acvtivity decreased with the increase of the concentration of Se4＋;if the concentration was lower than 0.01 μmol/L POD activity decreased with the increase of the concentration;when the concentration was lower than 0.005 μmol/L Se4＋ the content of MDA decreased as concentration increased.By polyacrylancide gel electrophoresis（PAGE）,different concentrations of Se4＋ did not induce the expression of special strips for POD isoenzymes,but the activities were different from each other,some strips disappeared.

关 键 词：硒 抗氧化酶系统 同功酶 蚕豆 

分 类 号：R963[医药卫生—微生物与生化药学] Q949.32[医药卫生—药理学]