培养时间对玉米灰斑病菌毒素活性的影响  

The Effect of Culture Time on Activity of Cercospora zeae-maydis Toxins

作　　者：王桂清[1,2] 张涛[2] 孙华[2] 徐秀德[1] 董怀玉[1] 

机构地区：[1]辽宁省农业科学院,沈阳110161 [2]聊城大学农学院,山东聊城252059

出　　处：《中国农学通报》2010年第9期307-310,共4页Chinese Agricultural Science Bulletin

基　　金：山东省中青年科学家科研奖励基金(2006BS06014);山东省教育厅科学技术项目(J06N01)

摘　　要：为了优化玉米灰斑病菌的产毒条件,采用抑制胚根伸长法测定了培养时间对玉米灰斑病菌毒素产量和活性的影响。结果表明,以Fries培养液为基质,静止培养15d时,毒素的产量最高,为38.477mg/mL,毒素的活性最强,对玉米胚根生长的抑制中浓度为18.012%。说明Fries培养液中静止培养15d最适合玉米灰斑病菌毒素的产生。The effects of culture time on production and activity of Cercospora zeae-maydis toxins have been studied in this article. The mensuration of inhibition of radicle growth was used to examine the activity of the toxins. The results showed that the maximum production and the strongest inhibitory activity of Cercospora zeae-maydis toxins were obtained when it was statically cultured in the Fries nutrient fluid for 15 days. The maximum production of the toxins was 38.477 mg/mL. The median inhibitory concentration on maize radicle growth of the toxins was 18.012%. This indicated that the statically cultured in the Fries nutrient fluid for 15 days was the most fitting formation conditions of Cercospora zeae-maydis toxins. The results of this study will provide the foundation for optimization of formation conditions of Cercospora zeae-maydis toxins.

关 键 词：玉米灰斑病菌 毒素 培养时间 毒素产量 抑制率 

分 类 号：S435.131.49[农业科学—农业昆虫与害虫防治]